Technical Data
Media
McCoy’s 5A Medium w/L-Glutamine, HEPES (Powder)
M2701
Cell Culture Grade
Storage RT    Shipping RT
Components shown as mg/liter
InorganicSalts:
CalciumChloride•2H2O132.5
MagnesiumSulfate97.67
PotassiumChloride400
SodiumChloride6460
Sodium Phosphate•H2O Mono580
AminoAcids:
L-Alanine13.36
L-Arginine•HCl42.14
L-Asparagine, mono45.03
L-Aspartic Acid19.97
LCysteine•HCl•H2O35.14
L-Glutamic Acid22.07
L-Glutamine219.2
Glutathione0.5
Glycine7.51
L-Histidine•HCl•H2O20.96
Hydroxy-L-proline19.67
L-Isoleucine39.36
L-Leucine39.36
L-Lysine•HCl36.54
L-Methionine14.92
L-Phenylalanine16.52
L-Proline17.27
L-Serine26.28
L-Threonine17.87
L-Tryptophan3.06
L-Tyrosine18.12
L-Valine17.57
Vitamins:
Ascorbic Acid0.5
D-Biotin0.2
D-PantothenicAcid•Ca0.2
Choline Chloride5
FolicAcid10
myo-Inositol36
Niacin0.5
Nicotinamide0.5
p-AminobenzoicAcid1
Pyridoxal•HCl0.5
Pyridoxine•HCl0.5
Riboflavin0.2
Thiamine•HCl0.2
VitaminB-122
Other:
D-Glucose3000
HEPES FreeA cid5958
Peptone600
Pheno lRed ,Sodium10
Total:18g/L




Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

1. McCoy, T.A., Maxwell, M. and Kruse, P.F., Biol. Med. 100:55-118 (1959) 2. Patterson, M.K. and Dell’orco, R.T., Tissue Culture Association Manual.4:737-740 (1978) 3. Hsu, T.C. and Kellogg, D.S., J.N.C.I. 25:221-231 (1960) 4. Iwakata, S. and Grace, J.T., New York State Journal of Med. 64,:279-2282 (1964) 5. Morton, H.J., In Vitro. 6:89 (1970)