Technical Data
Media
McCoy’s 5A Medium, Iwakata & Grace, w/L-Glutamine, w/o Sodium Bicarbonate (Powder)
M2702
Cell Culture Grade
Storage RT/4°C    Shipping RT
Components shown as mg/liter
InorganicSalts:
Calcium Chloride•2H2O132.5
Magnesium Sulfate97.68
Potassium Chloride400.0
Sodium Chloride6460.0
Sodium Phosphate•H2O Mono 580.0
AminoAcids:
L-Alanine13.36
L-Arginine•HCl42.14
L-Asparagine, mono45.03
L-Aspartic Acid19.97
L Cysteine•HCl•H2O35.14
L-Glutamic Acid22.07
L-Glutamine219.2
Glutathione0.50
Glycine7.51
L-Histidine•HCl•H2O20.96
Hydroxy-L-proline19.67
L-Isoleucine39.36
L-Leucine39.36
L-Lysine•HCl36.54
L-Methionine14.92
L-Phenylalanine16.52
L-Proline17.27
L-Serine26.28
L-Threonine17.87
L-Tryptophan3.06
L-Tyrosine18.12
L-Valine17.57
Vitamins:
p-Aminobenzoic Acid1.00
Ascorbic Acid0.50
D-Biotin0.20
Choline Chloride5.00
Vitamin B122.00
Folic Acid10.00
myo-Inositol36.00
Niacin 0.50
Nicotinamide0.50
D-Pantothenic Acid•Ca0.20
Pyridoxal•HCl0.50
Pyridoxine•HCl0.50
Riboflavin0.20
Thiamine•HCl0.20
Other:
D-Glucose3000.0
Peptone600.0
Phenol Red, Sodium10.00
Total:12.0g/L



Directions per Liter: Dissolve 12g in 800-900ml of ddH2O stirring gently until completely solubilized. Add 2.2g/L sodium bicarbonate. Adjust pH of the medium to the desired level. Add additional water to bring the solution to1L Filter-sterilize using a 0.22 micron membrane filter. Aliquot into sterile containers. Do not autoclave. Contains heat-labile compounds that can be damaged with autoclaving.

Storage and Stability: Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.

Appearance: Off-white to light orange, homogeneous, free flowing powder

Solubility: Yellow, clear, complete

pH: As Reported

Endotoxin: 1EU/ml


Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

1. McCoy, T.A., Maxwell, M. and Kruse, P.F., Biol. Med. 100:55-118 (1959) 2. Patterson, M.K. and Dell’orco, R.T., Tissue Culture Association Manual.4:737-740 (1978) 3. Hsu, T.C. and Kellogg, D.S., J.N.C.I. 25:221-231 (1960) 4. Iwakata, S. and Grace, J.T., New York State Journal of Med. 64,:279-2282 (1964) 5. Morton, H.J., In Vitro. 6:89 (1970)