Technical Data
Media
McCoy’s 5A Medium w/L-Glutamine w/o Phenol Red (Powder)
M2704
Cell Culture Grade
Storage RT/4°C    Shipping RT
Components shown as g/Liter
Inorganic Salts
Calcium Chloride•2H2O0.1324
Magnesium Sulfate0.0976
Potassium Chloride0.40
Sodium Chloride6.46
Sodium Phosphate Monobasic0.504
Amino Acids
L-Alanine0.01336
L-Arginine•HCl0.04214
L-Asparagine•H2O0.04503
L-AsparticAcid0.01997
L Cysteine, Free base0.02424
L-Glutamic Acid , Sodium0.02207
L-Glutamine0.21915
Glycine0.00751
L-Histidine•HCl•H2O0.02096
Hydroxy-L-proline0.01967
L-Isoleucine0.03936
L-Leucine0.03936
L-Lysine0.03654
L-Methionine0.01492
L-Phenylalanine0.01652
L-Proline0.01727
L-Serine0.02628
L-Threonine0.01787
L-Tryptophan0.00306
L-Tyrosine•2Na•2H2O0.0261
L-Valine0.01757
Vitamins
P-Aminobenzoic Acid0.001
Ascorbic Acid•Na0.0005625
D-Biotin0.0002
Choline Chloride0.005
Vitamin B-120.002
Folic Acid0.01
myo-Inositol0.036
Nicotinic Acid0.0005
Niacinamide0.0005
D-Pantothenic Acid•Ca0.0002
Pyridoxal•HCl0.0005
Pyridoxine•HCl0.0005
Riboflavin0.0002
Thiamine•HCl0.0002
Other
D-Glucose3.00
Glutathione0.0005
Peptone0.60
Total11.9g/liter



Directions per Liter: Dissolve 11.9g in 800-900ml of ddH2O stirring gently until completely solubilized. If required, add sodium bicarbonate. Adjust pH of the medium to the desired level. Add additional water to bring the solution to1L Filter-sterilize using a 0.22 micron membrane filter. Aliquot into sterile containers. Do not autoclave. Contains heat-labile compounds that can be damaged with autoclaving.

Storage and Stability: Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.

Appearance: Pale Yellow to orange, homogeneous, free flowing powder.

Solubility: Pale orange, clear, complete

pH: 4.8-5.5

Endotoxin: 1EU/ml


Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

1. McCoy, T.A., Maxwell, M. and Kruse, P.F., Biol. Med. 100:55-118 (1959)
2. Patterson, M.K. and Dell’orco, R.T., Tissue Culture Association Manual.4:737-740 (1978)
3. Hsu, T.C. and Kellogg, D.S., J.N.C.I. 25:221-231 (1960)
4. Iwakata, S. and Grace, J.T., New York State Journal of Med. 64,:279-2282 (1964)
5. Morton, H.J., In Vitro. 6:89 (1970)