Technical Data
Media
McCoy’s 5A Medium, Iwakata & Grace, w/o L-Glutamine (Powder)
M2705
Cell Culture Grade
Storage RT    Shipping RT
Components shown as mg/liter
Inorganic Salts:
CalciumChloride•2H2O132.47
MagnesiumSulfate97.68
PotassiumChloride400
SodiumChloride6460
Sodium Phosphate Mono•H2O580
Amino Acids:
L-Alanine13.36
L-Arginine•HCl42.14
L-Asparagine,mono45.03
L-Aspartic Acid19.97
LCysteine•HCl•H2O35.14
L-Glutamic Acid22.07
Glutathione0.5
Glycine7.51
L-Histidine•HCl•H2O20.96
Hydroxy-L-proline19.67
L-Isoleucine39.36
L-Leucine39.36
L-Lysine•HCl36.54
L-Methionine14.92
L-Phenylalanine16.52
L-Proline17.27
L-Serine26.28
L-Threonine17.87
L-Tryptophan3.06
L-Tyrosine18.12
L-Valine17.57
Vitamins:
L-Ascorbic Acid0.5
D-Biotin0.2
D-Pantothenic Acid•Ca0.2
Choline Chloride5
FolicAcid10
myo-Inositol36
Niacin0.5
Nicotinamide0.5
P-Aminobenzoic Acid1
Pyridoxal•HCl0.5
Pyridoxine•HCl0.5
Riboflavin0.2
Thiamine•HCl0.2
Vitamin B-122
Other:
D-Glucose3000
Peptone600
Phenol Red ,Sodium10
Total:11.81g/L



Directions per Liter: 1. Add 900ml distilled/deionized water (25ºC) to a 1 liter container.2. Weigh 11.8g into container, stirring gently until completely solubilized. Heat gently with stirring.

3. Add 2.0g/Liter NaHCO3 with stirring. 4. Adjust pH to ± 0.1-0.3 pH units with 1N HCl or 1N NaOH.5. q.s. to final 1 Liter volume.6. Sterilize with 0.2µ membrane filter. Do not autoclave since heat-labile vitamins will lose their activity.7. Aliquot into sterile containers, store at 0-5ºC. Keep away from light.Do not autoclave since heat-labile vitamins will lose their activity.7. Aliquot into sterile containers, store at 0-5ºC. Keep away from light.


Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

1. McCoy, T.A., Maxwell, M. and Kruse, P.F., Biol. Med. 100:55-118 (1959)
2. Patterson, M.K. and Dell’orco, R.T., Tissue Culture Association Manual.4:737-740 (1978)
3. Hsu, T.C. and Kellogg, D.S., J.N.C.I. 25:221-231 (1960)
4. Iwakata, S. and Grace, J.T., New York State Journal of Med. 64,:279-2282 (1964)
5. Morton, H.J., In Vitro. 6:89 (1970)