Technical Data
Media
MCDB 110 Diploid Fibroblast-like Cell Medium (Powder)
M2720
Cell Culture Grade
Storage RT/4°C    Shipping RT
Components shown as mg/liter
Inorganic Salts:
Ammonium Metavandate0.000585
Ammonium Molybdate0.00124
Calcium Chloride•2H20147.00
Cupric Sulfate •5H200.00025
Ferrous Sulfate•7H201.39
Magnesium Sulfate120.38
Manganese Sulfate•5H2O0.000151
Nickel Chloride Hexahydrate0.00012
Potassium Chloride372.75
Sodium Chloride6546.00
Sodium Metasilicate•9H2O0.1421
Sodium Phosphate Dibasic425.94
Sodium Selenite0.0038
Stannous Chloride Dihydrate0.000113
Zinc Sulfate•7H2O0.144
Amino Acids:
L-Alanine8.90
L-Arginine HCl210.70
L-Asparagine H2O15.00
L-Aspartic Acid13.31
L-Cysteine HCl H2O8.78
L-Glutamic Acid14.71
L-Glutamine365.30
Glycine22.52
L-Histidine HCl H2O20.97
L-Isoleucine3.94
L-Leucine13.12
L-Lysine HCl36.54
L-Methionine4.48
L-Phenylalanine4.96
L-Proline34.53
L-Serine10.51
L-Threonine11.91
L-Tryptophan2.04
L-Tyrosine7.84
L-Valine11.72
Vitamins:
Biotin0.00733
Choline Chloride13.96
Vitamin B120.136
Folinic Acid, Calcium0.000602
Myo-Inositol18.02
Nicotinamide6.11
D-Pantothenic Acid•Ca0.2383
Pyridoxine•HCl0.0617
Riboflavin0.113
Thiamine•HCl0.337
Other:
Adenine HCl1.72
Thymidine0.0727
D-Glucose720.64
HEPES Free Acid5958.00
Thioctic Acid0.00206
Phenol Red, Sodium1.242
Putrescine•2HCl0.0001611
Sodium Pyruvate110.00
Total15.3g/liter
Basal Medium MCDB 110 is specifically formulated for the cultivation of diploid fibroblast-like cells.

MCDB 105 and 110 are modifications of MCDB 104 medium. These modifications are optimized for long-term survival and rapid clonal growth of human diploid fibroblast-like cells (WI-38, MRC-5, IMR-90) and of low-passage human foreskin fibroblasts using FBSP or hormone and growth factor supplements.

Supplement with growth factors or fetal calf serum (0.2-2.0% dialyzed).


Directions per Liter: Dissolve 15.3g in 800-900ml of ddH2O stirring gently until completely solubilized. Add sodium bicarbonate if required. Adjust pH of the medium to the desired level. Add additional water to bring the solution to 1L. Filter-sterilize using a 0.22 micron membrane filter. Aliquot into sterile containers. Do not autoclave. Contains heat-labile compounds that can be damaged with autoclaving.

Storage and Stability: Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.

Appearance: White to light orange, homogeneous, free flowing powder

Solubiliy: Pale yellow to orange, clear, complete

pH: As Reported

Endotoxin: 1EU/ml


Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.