Technical Data
M2750-11B
MCP2, Recombinant, Human (Macrophage/Monocyte Chemotactic Protein 2, Chemokine C-C Motif Ligand 8, CCL8, HC14, Monocyte Chemotactic Protein 2, Small Inducible Cytokine A8 Precursor, SCYA8, SCYA10)
5ug
Molecular Biology Storage: -20CShipping: Blue Ice
Source:
Recombinant corresponding to human MCP2 expressed in E. coli.

Molecular Weight:
~8.5kD

Endotoxin: ~0.1ng/ug

Biological Activity:
Determined by measuring the dose dependent mobilization of intracellular calcium (calcium flux) with human THP-1 cells. Significant calcium mobilization is observed with >500ng/ml of recombinant human MCP-2. Human MCP-2 also induces dose dependent chemotaxis of human THP-1 cells with an ED50=30-100ng/ml. The optimal concentration should be determined for each specific application.

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile dH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.


Molecular Weight:
~8.5kD
Purity: ~95% (SDS-PAGE)
Concentration: Not determined
Form: Supplied as a lyophilized powder. BSA free. Reconstitute with sterile dH2O to 0.1-0.5mg/ml.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
1. Van Damme, J., P. Proost, J.P. Lenaerts, and G. Opdenakker (1992) Structural and functional identification of two human, tumor-derived monocyte chemotactic proteins (MCP-2 and MCP-3) belonging to the chemokine family. J. Exp. Med. 176:59-65. 2. Cross, A.K., V. Richardson, S.A. Ali, I. Palmer, D.D. Taub, and R.C. Rees (1997) Migration responses of human monocytic cell lines to alpha- and beta-chemokines. Cytokine 9:521-528. 3. Gong, X., W. Gong, D.B. Kuhns, A. Ben-Baruch, O.M. Howard, and J.M. Wang (1997) Monocyte chemotactic protein-2 (MCP-2) uses CCR1 and CCR2B as its functional receptors. J. Biol. Chem. 272:11682-11685. 4. Gong, W., O.M. Howard, J.A. Turpin, M.C. Grimm, H. Ueda, P.W. Gray, C.J. Raport, J.J. Oppenheim, and J.M. Wang (1998) Monocyte chemotactic protein-2 activates CCR5 and blocks CD4/CCR5-mediated HIV-1 entry/replication. J. Biol. Chem. 273:4289-4292.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.