Technical Data
M2750-11B
MCP2, Recombinant, Human (Macrophage/Monocyte Chemotactic Protein 2, Chemokine C-C Motif Ligand 8, CCL8, HC14, Monocyte Chemotactic Protein 2, Small Inducible Cytokine A8 Precursor, SCYA8, SCYA10)
5ug
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Technical Data |
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M2750-11B |
MCP2, Recombinant, Human (Macrophage/Monocyte Chemotactic Protein 2, Chemokine C-C Motif Ligand 8, CCL8, HC14, Monocyte Chemotactic Protein 2, Small Inducible Cytokine A8 Precursor, SCYA8, SCYA10) |
5ug |
| Growth Factors, Cytokines | Storage: -20°CShipping: Blue Ice |
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Source: Recombinant corresponding to human MCP2 expressed in E. coli. Molecular Weight: ~8.5kD Endotoxin: ~0.1ng/ug Biological Activity: Determined by measuring the dose dependent mobilization of intracellular calcium (calcium flux) with human THP-1 cells. Significant calcium mobilization is observed with >500ng/ml of recombinant human MCP-2. Human MCP-2 also induces dose dependent chemotaxis of human THP-1 cells with an ED50=30-100ng/ml. The optimal concentration should be determined for each specific application. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile dH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Molecular Weight: ~8.5kD |
Purity: ~95% (SDS-PAGE) Concentration: Not determined Form: Supplied as a lyophilized powder. BSA free. Reconstitute with sterile dH2O to 0.1-0.5mg/ml. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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1. Van Damme, J., P. Proost, J.P. Lenaerts, and G. Opdenakker (1992) Structural and functional identification of two human, tumor-derived monocyte chemotactic proteins (MCP-2 and MCP-3) belonging to the chemokine family. J. Exp. Med. 176:59-65. 2. Cross, A.K., V. Richardson, S.A. Ali, I. Palmer, D.D. Taub, and R.C. Rees (1997) Migration responses of human monocytic cell lines to alpha- and beta-chemokines. Cytokine 9:521-528. 3. Gong, X., W. Gong, D.B. Kuhns, A. Ben-Baruch, O.M. Howard, and J.M. Wang (1997) Monocyte chemotactic protein-2 (MCP-2) uses CCR1 and CCR2B as its functional receptors. J. Biol. Chem. 272:11682-11685. 4. Gong, W., O.M. Howard, J.A. Turpin, M.C. Grimm, H. Ueda, P.W. Gray, C.J. Raport, J.J. Oppenheim, and J.M. Wang (1998) Monocyte chemotactic protein-2 activates CCR5 and blocks CD4/CCR5-mediated HIV-1 entry/replication. J. Biol. Chem. 273:4289-4292.
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