Technical Data
MCP2, Recombinant, Mouse (Macrophage/Monocyte Chemotactic Protein 2, Chemokine C-C Motif Ligand 8, CCL8, HC14, Monocyte Chemotactic Protein 2, Small Inducible Cytokine A8 Precursor, SCYA8, SCYA10)
Molecular Biology Storage: -20CShipping: Blue Ice
MCP-2 (Monocyte Chemoattractant Protein 2), otherwise known as CCL8, is a CC chemokine originally isolated from the human osteosarcoma cell line MG-63, which is expressed at highest levels in peripheral blood cells and the small intestine. MCP-2 acts as a chemoattractant for monocytes, lymphocytes, eosinophils and basophils, and signals through binding to the common MCP receptor, chemokine (C-C motif) receptor 2 (CCR2), and also CCR1 and CCR3. Proteolytic cleavage of MCP-2 into the NH2-terminally truncated form, MCP-2 (6-76), results in the loss of chemotactic activity, forming a functional CC chemokine inhibitor, which may be important during inflammatory responses.

E. coli

Suitable for use in Functional Assays. Other applications not tested.

Biological Activity:
Determined by its ability to chemoattract human peripheral blood monocytes using a concentration range of 10-100ng/ml.

Endotoxin: 1EU/ug

Molecular Weight: 8.5kD (74 amino acid residues)

Storage and Stability:
Lyophilized powder may be stored at -20C. Reconstitute with sterile buffer or ddH2O. Aliquot and store at -20C. Reconstituted product is stable for 6 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Source: E. coli
Purity: 98% (SDS PAGE and HPLC).
Concentration: ~0.1mg/ml
Form: Supplied as a lyophilized powder protein. Reconstitute with 200ul sterile dH20 PBS.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.