Technical Data
MCP3, Recombinant, Human (Macrophage/Monocyte Chemotactic Protein 3, MARC)
Molecular Biology Storage: -20CShipping: RT
Recombinant Human MCP-3 is a non-glycosylated, Polypeptide chain containing 76 amino acids and having a molecular mass of 9011 Dalton.

AA Sequence:
The sequence of the first five N-terminal amino acids was determined and was found to be Gln-Pro-Val-Gly-Ile.

Biological Activity:
MCP-3 is fully biologically active when compared to standard. The specific activity as determined by the ability of MCP-3 to chemoattract human peripheral blood at 10-100ng/ml.

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute, aliquot and store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Source: E. coli
Purity: 98% by RP-HPLC, FPLC, or reducing/non-reducing SDS-PAGE Silver Stain. Chromatographically purified. Endotoxin: 0.1ng/ug (IEU/ug) of recombinant Monocyte Chemotactic Protein-3
Form: Supplied as a lyophilized powder. No additives. Reconstitute with sterile dH2O, 0.1% HSA or BSA to > 0.1mg/ml.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
1. MCP-1, MCP-2 and MCP-3 expression in multiple sclerosis lesions: an immunohistochemical and in situ hybridization study. McManus C, et al., J Neuroimmunol 1998 Jun 1;86(1):20-9 2. Monocyte chemotactic protein 4 (MCP-4), a novel structural and functional analogue of MCP-3 and eotaxin. Uguccioni M, et al., J Exp Med 1996 May 1;183(5):2379-84 3. Chemical synthesis, purification and folding of the human monocyte chemotactic proteins MCP-2 and MCP-3 into biologically active chemokines. Proost P, et al., 1995 Feb;7(2):97-104 4. Actions of the chemotactic cytokines MCP-1, MCP-2, MCP-3, RANTES, MIP-1 alpha and MIP-1 beta on human monocytes. Uguccioni M, et al., Eur J Immunol 1995 Jan;25(1):64-8 5. Human monocyte chemotactic protein-3 (MCP-3): molecular cloning of the cDNA and comparison with other chemokines. Opdenakker G, Froyen G, Proost P, Biochem Biophys Res Commun 1993 Mar 15;191(2):535-42 6. Minty A, et al., Eur Cytokine Netw 1993 Mar-Apr;4(2):99-110.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.