		Technical Data
M2755-25	MCSP (Melanoma-associated Chondroitin Sulfate Proteoglycan, Chondroitin Sulfate Proteoglycan 4 (Melanoma Associated), Chondroitin Sulfate Proteoglycan 4, Chondroitin Sulfate Proteoglycan NG2, CSPG4, MCSPG, MEL-CSPG, Melanoma Chondroitin Sulfate Proteoglyc

Description:
Proteoglycans (PGs) are a family of proteins composed of different polypeptide chains containing glycosaminoglycan (GAG) modifications. They vary in their cellular locations and have diverse structure and functions. MCSP is a proteoglycan found in the nervous system, comprising a large integral membrane PG with a core protein of 300kD and at least one covalently attached chondroitin sulfate (CS) GAG chain. In the CNS this protein is expressed mainly on the surfaces of developing and adult oligodendrocyte precursor cells, but is also associated with developing chondrocytes, cardiomyocytes, pericytes and several human tumors.

Applications:
Suitable for use in Flow Cytometry, Western Blot, Immunoprecipitation, Immunofluorescence and Immunohistochemistry. Other applications not tested.

Recommended Dilutions:
Immunohistochemistry: Paraffin and frozen sections
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Type	Isotype	Clone	Grade
Mab	IgG1,k	8L721	Purified

Size		Storage	Shipping	Source	Host
100ug		-20°C	Blue Ice	Human	Mouse

Concentration:

~1mg/ml

Immunogen:

Tissue/cell preparation (Human - a 375P cell crude extract). Cellular Localization: Cell membrane

Purity:

Purified

Form

Supplied as a liquid in PBS. No preservative added.

Specificity:

Recognizes human MCSP at ~240kD.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Kinney HC, Haynes RL, Xu G, Andiman SE, Folkerth RD, Sleeper LA, Volpe JJ. Neuron deficit in the white matter and subplate in periventricular leukomalacia. Ann Neurol;71(3):397-406. 2012 Mar. (ICC, IF, Human).