Technical Data
M2755-25
MCSP (Melanoma-associated Chondroitin Sulfate Proteoglycan, Chondroitin Sulfate Proteoglycan 4 (Melanoma Associated), Chondroitin Sulfate Proteoglycan 4, Chondroitin Sulfate Proteoglycan NG2, CSPG4, MCSPG, MEL-CSPG, Melanoma Chondroitin Sulfate Proteoglyc
Description:
Proteoglycans (PGs) are a family of proteins composed of different polypeptide chains containing glycosaminoglycan (GAG) modifications. They vary in their cellular locations and have diverse structure and functions. MCSP is a proteoglycan found in the nervous system, comprising a large integral membrane PG with a core protein of 300kD and at least one covalently attached chondroitin sulfate (CS) GAG chain. In the CNS this protein is expressed mainly on the surfaces of developing and adult oligodendrocyte precursor cells, but is also associated with developing chondrocytes, cardiomyocytes, pericytes and several human tumors.

Applications:
Suitable for use in Flow Cytometry, Western Blot, Immunoprecipitation, Immunohistochemistry and Immunofluorescence/Immunocytochemistry. Other applications not tested.

Recommended Dilutions:
Flow Cytometry: 1:400
Western Blot: 1:1000
Immunofluorescence (IC): 1:100
Immunoprecipitation: 1:10-1:100
Immunohistochemistry(Paraffin/frozen): 1:200
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG1,k8L721Affinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanMouse
Concentration:
~1mg/ml
Immunogen:
A 375P cells crude extract. Cellular Localization: Cell membrane
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in Tri-glycine, 150mM sodium chloride, 0.05% sodium azide.
Specificity:
Recognizes human MCSP at ~300kD.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Kinney HC, Haynes RL, Xu G, Andiman SE, Folkerth RD, Sleeper LA, Volpe JJ. Neuron deficit in the white matter and subplate in periventricular leukomalacia. Ann Neurol;71(3):397-406. 2012 Mar. (ICC, IF, Human).