Technical Data
MDM2 (Murine Double Minute-2)
The MDM2 (murine double minute 2) proto-oncogene was originally identified as an amplified gene in a mouse tumor cell line. Overexpression of MDM2 produces tumors in athymic mice and binds to and inactivates the transcriptional activity of the p53 protein. p53 was identified as the major target for MDM2 during embryonic development. The MDM2 gene itself is a transcriptional target for p53, and induction of p53 transcriptional activity results in increased MDM2 mRNA and protein levels. It appears that a MDM2 p53 feedback loop serves to keep the growth suppressive functions of p53 in check during the normal cell cycle. MDM2 regulates cell proliferation via p53-independent pathways and can interact with Rb, E2F-1 and DP1. Because MDM2 can bind to and inactivate the transcriptional activity of p53, Overexpression has been detected in a variety of human tumors, and appears to result from gene amplification, increased transcript level, and enhanced translation.

Suitable for use in Immunohistochemistry and Immunofluorescence. Other applications not tested.

Recommended Dilution:
Immunohistochemistry: 1:50-1:100
Immunofluorescence: 1:100-1:200
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
PabIgGAffinity Purified
100ug-20CBlue IceHumanRabbit
Synthesized non-phosphopeptide derived from human MDM2 around the phosphorylation site of serine 166 (A-I-SP-E-T).
Purified by immunoaffinity chromatography.
Supplied as a liquid in PBS (without Mg2+ and Ca2+), pH 7.4, 150mM sodium chloride, 0.02% sodium azide, 50% glycerol.
Recognizes endogenous levels of total human MDM2 protein.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.