		Technical Data
M2825-13C	MDR Protein (PE) (MRP1, Multidrug resistance-associated protein 1, ABCC1, ATP-binding cassette sub-family C member 1, Leukotriene C(4) transporter)

Description:
MRP1 is a 190kD transmembrane phosphoglycoprotein overexpressed in various human multidrug resistant tumor cell lines and tumors (1-5).

Applications:
Suitable for use in Western Blot, Immunoprecipitation, Flow Cytometry, Immunocytochemistry and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 1:100-1:500 (Anti mouse IgG labeled with HRP).
Immunocytochemistry: 1:20-1:50. [PBS, 0.5% paraformaldehyde-fixed or 70% methanol-fixed cytospin preparations (2)].
Immunohistochemistry: FFPE sections following antigen retrieval by microwave heating in citrate (5).
Flow Cytometry: 1:20. Cells fixed in PBS, 0.5% paraformaldehyde for 30 minutes, followed by anti-mouse IgG labeled with FITC. Note: Block and wash with solution containing 0.1% Tween.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C before opening. DO NOT FREEZE! Stable at 4°C as an undiluted liquid. Dilute only prior to immediate use. Freezing R-Phycoerythrin (PE) conjugates will result in a substantial loss of activity. Protect from light.

Type	Isotype	Clone	Grade
Mab	IgG1	3H406	Affinity Purified

Size		Storage	Shipping	Source	Host
100ul		4°C Do not freeze	Blue Ice	Human	Mouse

Concentration:

Not determined

Immunogen:

Non-denatured membranes prepared from the human small cell lung cancer cell line, H69AR, which highly overexpresses MRP1(1-3).

Purity:

Purified by affinity chromatography.

Form

Supplied as a liquid in PBS, pH 7.2. Labeled with R-Phycoerythrin (PE).

Specificity:

Recognizes human MDR protein with a defined linear epitope (aa918-924) of MRP1. Does not crossreact with human MDR1, MDR3, MRP2, MRP3, MRP4, MRP5 or MRP6 gene products. Does not crossreact with mouse mrp1.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1) Cole, S.P.C. et al. (1992). Science 258: 1650-1654. 2) Hipfner, D.R. et al. (1999). Biochim. Biophys. Acta 1461: 359-376. 3) Hipfner, D.R. et al. (1994). Cancer Res. 54: 5788-5792. 4) Hipfner, D.R. et al. (1996). Cancer Res. 56: 3307-3314. 5) Wright, S. et al. (1998). Clin. Cancer Res. 4: 2279-2289.