Technical Data
M2825-14
MDR1 (Multidrug Resistance Protein 1, ATP-binding Cassette Sub-family B Member 1, P-glycoprotein 1, CD243, ABCB1, PGY1)
Description:
P-glycoprotein is encoded by the MDR1 (ABCB1) gene. It is a member of the ABC superfamily of ATP-binding cassette transporters. Expression of P-glycoprotein is associated with ATP-dependent transmembrane efflux of chemically diverse compounds from the cell. It is the major component of multidrug resistance in human tumors.

Applications:
Suitable for use in Western Blot, Immunohistochemistry and Immunocytochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 0.5-1ug/ml
Immunohistochemistry (paraffin, frozen): 1-2ug/ml. Boiling sections is required
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG110K274Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanMouse
Concentration:
~0.1mg/ml (after reconstitution)
Immunogen:
Mixture of human and hamster drug-resistant whole cells and crude plasma membranes.
Purity:
Purified
Form
Supplied as a lyophilized powder from 1.2% sodium acetate, 2% BSA, 0.01% sodium azide. Reconstitute with 1ml sterile PBS.
Specificity:
Recognizes human MDR1.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Callen D.F., et al., Human Genet 77:142-144 (1987) 2. de Lannoy, I. et al., Biochem Biophys res Commun 189: 554-557 (1992)