Technical Data
M2825-14A
MDR1 (Multi Drug Resistance Protein 1, ABC20, ATP Binding Cassette Subfamily B (MDR/TAP) Member 1, ABCB1, CD243, CLCS, GP170, P-Glycoprotein, P-gp, P Glycoprotein 1, PGY1)
Description:
The selection of cell lines exhibiting multi-drug resistance offers the opportunity to investigate the mechanism of pleiotropic drug resistance in vitro. Reportedly useful in making a distinction between drug-sensitive and drug-resistant CHO cells.

Applications:
Suitable for use in Flow Cytometry, Immunofluorescence, Western Blot, and Immunohistochemistry. Other applications have not been tested.

Recommended Dilution:
Western Blot: 1-2 ug/ml for 2 hours at room temperature.
Immunohistochemistry: acetone fixed frozen.
Optimal dilutions to be determined by the researcher.

Positive Control:
Drug resistant Chinese Hamster Ovary (CHO) cells.

Cellular Localization:
Cell membrane

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG1,k0.N.504Affinity Purified
SizeStorageShippingSourceHost
250ul4°C (-20°C Glycerol)Blue IceHamsterMouse
Concentration:
~0.1mg/ml
Immunogen:
Colchicine-resistant viable Chinese Hamster Ovary (CHO) cells, followed by purified P170 glycoprotein from CHO cells.
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in 10mM PBS, pH 7.4, with 0.2% BSA and 0.09% sodium azide.
Specificity:
Recognizes a membrane glycoprotein of 170kD in drug-resistant chinese hamster ovary (CHO) cells, which is identified as multi-drug resistance(MDR) associated p-glycoprotein (p170). Does not react with human. Shows no crossreactivity with parent drug-sensitive CHO cells.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.