Technical Data
M2825-14B
MDR1 (Multi Drug Resistance Protein 1, ABC20, ATP Binding Cassette Subfamily B (MDR/TAP) Member 1, ABCB1, CD243, CLCS, GP170, P-Glycoprotein, P-gp, P Glycoprotein 1, PGY1)
Description:
Shows no crossreactivity with the human MDR3 product. Its epitope localizes in the extracellular amino terminal half of P-glycoprotein (at the third extracellular loop of the molecule). This epitope is resistant to formalin fixation and periodate oxidation1 and is different from those of other commonly known Mabs to P-glycoprotein, such as C219, JSB-1, and MRK16, which recognize an intracellular and extracellular epitope of P-glycoprotein, respectively. Detects soluble P-glycoprotein in the cultured media of viable adriamycin-resistant cells, whereas those of the drug-sensitive parent cells contain no detectable level of soluble P-glycoprotein.2 Soluble P-glycoprotein is also detected in extracellular fluids of cancer patients, such as malignant ascites and serum. The Mr of soluble P-glycoprotein is the same as that of membrane bound P-glycoprotein.

Applications:
Suitable for use in Flow Cytometry, Immunofluorescence, Immunohistochemistry and Western Blot. Other applications have not been tested.

Recommended Dilutions:
Flow Cytometry: Fixed cells only.
Western Blot: 1-2ug/ml for 2hrs at RT
Immunohistochemistry: Formalin/paraffin
Optimal dilutions to be determined by the researcher.

Positive Control:
Drug resistant cells, Kidney or breast carcinoma.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG10.N.505Affinity Purified
SizeStorageShippingSourceHost
500ul-20CBlue IceHumanMouse
Concentration:
~0.2mg/ml
Immunogen:
Human and hamster drug-resistant viable cells followed by crude plasma membranes. Cellular Localization: Cell membrane. MW: 170kD. Epitope: Extracellular amino half
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide.
Specificity:
Recognizes soluble P-Glycoprotein in cultured media of viable adriamycin-resistant cells, whereas those of the drug-resistant parent cells contain no detectable level of soluble P-Glycoprotein. Species crossreactivity: human and chinese hamster. Does not react with mouse.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Chu TM, et. al. Hybridoma, 1993, 12(4):417-29. 2.Chu TM, et. al. Biochemical and Biophysical Research Communications, 1994, 203:506-12.