Technical Data
M2825-14E
MDR1 (P-Glycoprotein, Pgp170, MRP1, Multi Drug Resistance Associated Protein, ABCB1, ATP-binding cassette, sub-family B (MDR/TAP), member 1, ABC20, CD243, CD243 antigen, CLCS, GP170)
Description:
CD243, also known as MDR1 (multi-drug resistance protein 1) and P-glycoprotein, is a 170kD membrane-associated protein which functions as an ATP-dependent drug efflux pump with broad substrate specificity. CD243 is highly expressed in the kidney, liver, adrenal gland and intestine, suggesting a primary role in the secretion of metabolites into bile, urine and the lumen of the intestinal tract. CD243 also prevents the accumulation of toxic compounds in critical organs such as the brain, gonads, and bone marrow, and also functions in the placenta to protect the fetus. CD243 is rapidly up-regulated by chemotherapeutic drugs and is a well established mediator of multidrug resistance.

Applications:
Suitable for use in Immunohistochemistry (paraffin). Other applications not tested.

Recommended Dilution:
Immunohistochemistry (paraffin): 20ug/ml
Requires antigen retrieval using heat treatment for 20 minutes prior to staining of paraffin sections. Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
50ug-20CBlue IceHumanRabbit
Concentration:
~0.5mg/ml
Immunogen:
Synthetic peptide corresponding to cytoplasmic domain of human CD243, conjugated to KLH.
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.7, 0.09% sodium azide.
Specificity:
Recognizes human MDR1.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Marzolini, C. et al. (2004) Polymorphisms in human MDR1 (P-glycoprotein): recent advances and clinical relevance. Clin. Pharmacol. Ther. 75: 13-33.