Technical Data
M2825-14F
MDR1 (P-Glycoprotein, Pgp170, MRP1, Multi Drug Resistance Associated Protein, ABCB1, ATP-binding cassette, sub-family B (MDR/TAP), member 1, ABC20, CD243, CD243 antigen, CLCS, GP170)
Description:
Applications:
Suitable for use in Flow Cytometry, Immunofluorescence, Immunohistochemistry (frozen and paraffin), and Immunoprecipitation. Other applications not tested.

Recommended Dilution:
Flow Cytometry: 1:20-1:200; 10ul labels 10e6 cells in 100ul
Immunohistochemistry (paraffin): 1:200
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG2a9A183Affinity Purified
SizeStorageShippingSourceHost
200ug4°C/-20°C (No Glycerol)Blue IceHumanMouse
Concentration:
~1mg/ml
Immunogen:
Mouse Balb/c 3T3 fibroblasts transfected with human CD243 cDNA.
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide.
Specificity:
Recognizes human CD243. Species Crossreactivity: primate. Does not react with: mouse, rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Mechetner, E.B and Roninson, I.B. (1992) Efficient inhibition of P-glycoprotein-mediated multidrug resistance with a monoclonal antibody. P.N.A.S. 89: 5824-5828, 2. Park, S.W. et al. (2003) Analysis of P-glycoprotein-mediated membrane transport in human peripheral blood lymphocytes using the UIC2 shift assay. Cytometry Part A. 53A: 67-78, 3. Koziolek, M.J. et al. (2001) Expression of multidrug resistance P-glycoprotein in kidney allografts from cyclosporine A-treated patients. Kid. Int. 60: 156-166, 4. Beck, W.T. et al. (1996) Methods to detect P-glycoprotein-associated multidrug resistance in patients’ tumours: Consensus recommendations. Cancer. Res. 56: 3010-3020.