		Technical Data
M2825-14F1	MDR1 (P-Glycoprotein, Pgp170, MRP1, Multi Drug Resistance Associated Protein, ABCB1, ATP-binding cassette, sub-family B (MDR/TAP), member 1, ABC20, CD243, CD243 antigen, CLCS, GP170)

Description:
Applications:
Suitable for use in Flow Cytometry, Functional Assays, Immunofluorescence, Immunohistochemistry (frozen and paraffin), and Immunoprecipitation. Other applications not tested.

Recommended Dilution:
Flow Cytometry: 1:50-1:200; 10ul labels 10e6 cells in 100ul
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Type	Isotype	Clone	Grade
Mab	IgG2a	9A183	Affinity Purified

Size		Storage	Shipping	Source	Host
500ug		4°C/-20°C (No Glycerol)	Blue Ice	Human	Mouse

Concentration:

~1mg/ml

Immunogen:

Mouse Balb/c 3T3 fibroblasts transfected with human CD243 cDNA.

Purity:

Purified by Protein G affinity chromatography.

Form

Supplied as a liquid in PBS, pH 7.4. No preservative added.

Specificity:

Recognizes human CD243. Species Crossreactivity: primate. Does not react with: mouse, rat.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Mechetner, E.B and Roninson, I.B. (1992) Efficient inhibition of P-glycoprotein-mediated multidrug resistance with a monoclonal antibody. P.N.A.S. 89: 5824-5828, 2. Park, S.W. et al. (2003) Analysis of P-glycoprotein-mediated membrane transport in human peripheral blood lymphocytes using the UIC2 shift assay. Cytometry Part A. 53A: 67-78, 3. Koziolek, M.J. et al. (2001) Expression of multidrug resistance P-glycoprotein in kidney allografts from cyclosporine A-treated patients. Kid. Int. 60: 156-166, 4. Beck, W.T. et al. (1996) Methods to detect P-glycoprotein-associated multidrug resistance in patients’ tumours: Consensus recommendations. Cancer. Res. 56: 3010-3020.