Technical Data
M2825-14J
MDR1 (Multi Drug Resistance Protein 1, ABC20, ATP Binding Cassette Subfamily B (MDR/TAP) Member 1, ABCB1, CD243, CLCS, GP170, P-Glycoprotein, P-gp, P Glycoprotein 1, PGY1)
Description:
P-glycoprotein is encoded by the MDR1 (ABCB1) gene. It is a member of the ABC superfamily of ATP-binding cassette transporters. Expression of P-glycoprotein is associated with ATP-dependent transmembrane efflux of chemically diverse compounds from the cell. It is the major component of multidrug resistance in human tumors.

Applications:
Suitable for use in Immunohistochemistry (frozen and paraffin embedded tissue sections). Other applications not tested.

Recommended Dilution:
Immunohistochemistry: 1:40
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG2b3H407Purified
SizeStorageShippingSourceHost
100ug4C (-20C Glycerol)Blue IceHumanMouse
Concentration:
~1mg/ml
Immunogen:
Human MDR-transfected NIH3T3 cells
Purity:
Purified
Form
Supplied as a liquid in PBS, pH 7.6, 0.1% sodium azide.
Specificity:
Recognizes human P-glycoprotein.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Scheper, R.J., et al., Int. J. Cancer 42: 389 (1986). 2. Dalton, W.S.,et al., J. Clin. Oncol. 7: 415 (1989). 3. Tiirikainen, M., et al., Ann. Haematol. 65: 124 (1992).. 4. Grogan, T., et al., Blood 81: 490 (1993). 5. Itsubo, M., et al., Cancer 73: 298 (1994). 6. Toth, K., et al., Am. J. Pathol. 144: 227 (1994). 7. Gala, et al., J. Clin. Pathol. 47: 619 (1994). 8. Sinicrope, et al., Cancer 74: 2908 (1994). 9. Balaini, et al., New Engl. J. Med. 333: 1380 (1995). 10. Chevillard, et al., Cancer 77: 292 (1996). 11. Moran, et al., Eur. J. Cancer 33: 652 (1997).