		Technical Data
M2825-14K	MDR1 (Multi Drug Resistance Protein 1, ABC20, ATP Binding Cassette Subfamily B (MDR/TAP) Member 1, ABCB1, CD243, CLCS, GP170, P-Glycoprotein, P-gp, P Glycoprotein 1, PGY1)

Description:
P-glycoprotein is encoded by the MDR1 (ABCB1) gene. It is a member of the ABC superfamily of ATP-binding cassette transporters. Expression of P-glycoprotein is associated with ATP-dependent transmembrane efflux of chemically diverse compounds from the cell. It is the major component of multidrug resistance in human tumors.

Applications:
Suitable for use in Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Immunohistochemistry (Frozen and paraffin embedded): 1:40
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Type	Isotype	Clone	Grade
Mab	IgG2a	3H408	Affinity Purified

Size		Storage	Shipping	Source	Host
100ug		4°C (-20°C Glycerol)	Blue Ice	Human	Mouse

Concentration:

~1mg/ml

Immunogen:

Human MDR-transfected NIH3T3 cells.

Purity:

Purified by affinity chromatography.

Form

Supplied as a liquid in PBS, pH 7.6, 0.1% sodium azide.

Specificity:

Recognizes human P-glycoprotein.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Scheper, R.J., et al., Int. J. Cancer 42: 389 (1986). 2. Dalton, W.S.,et al., J. Clin. Oncol. 7: 415 (1989). 3. Tiirikainen, M., et al., Ann. Haematol. 65: 124 (1992).. 4. Grogan, T., et al., Blood 81: 490 (1993). 5. Itsubo, M., et al., Cancer 73: 298 (1994). 6. Toth, K., et al., Am. J. Pathol. 144: 227 (1994). 7. Gala, et al., J. Clin. Pathol. 47: 619 (1994). 8. Sinicrope, et al., Cancer 74: 2908 (1994). 9. Balaini, et al., New Engl. J. Med. 333: 1380 (1995). 10. Chevillard, et al., Cancer 77: 292 (1996). 11. Moran, et al., Eur. J. Cancer 33: 652 (1997).