Technical Data
MDR1 (Multi Drug Resistance Protein 1, ABC20, ATP Binding Cassette Subfamily B (MDR/TAP) Member 1, ABCB1, CD243, CLCS, GP170, P-Glycoprotein, P-gp, P Glycoprotein 1, PGY1)
P-glycoprotein is encoded by the MDR1 (ABCB1) gene. It is a member of the ABC superfamily of ATP-binding cassette transporters. Expression of P-glycoprotein is associated with ATP-dependent transmembrane efflux of chemically diverse compounds from the cell. It is the major component of multidrug resistance in human tumors.

Suitable for use in Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Immunohistochemistry (Frozen and paraffin embedded): 1:40
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
MabIgG2a3H408Affinity Purified
100ug4C (-20C Glycerol)Blue IceHumanMouse
Human MDR-transfected NIH3T3 cells.
Purified by affinity chromatography.
Supplied as a liquid in PBS, pH 7.6, 0.09% sodium azide.
Recognizes human P-glycoprotein.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Scheper, R.J., et al., Int. J. Cancer 42: 389 (1986). 2. Dalton, W.S.,et al., J. Clin. Oncol. 7: 415 (1989). 3. Tiirikainen, M., et al., Ann. Haematol. 65: 124 (1992).. 4. Grogan, T., et al., Blood 81: 490 (1993). 5. Itsubo, M., et al., Cancer 73: 298 (1994). 6. Toth, K., et al., Am. J. Pathol. 144: 227 (1994). 7. Gala, et al., J. Clin. Pathol. 47: 619 (1994). 8. Sinicrope, et al., Cancer 74: 2908 (1994). 9. Balaini, et al., New Engl. J. Med. 333: 1380 (1995). 10. Chevillard, et al., Cancer 77: 292 (1996). 11. Moran, et al., Eur. J. Cancer 33: 652 (1997).