Technical Data
M2825-14M
MDR1 (Multi Drug Resistance Protein 1, Multidrug Resistance Protein 1, ABC20, ATP Binding Cassette Subfamily B (MDR/TAP) Member 1, ABCB1, CD243, CLCS, GP170, MGC163296, P-Glycoprotein, P-gp, P Glycoprotein 1, PGY1)
Description:
P Glycoprotein belongs to the MDR/TAP subfamily of ATP-binding cassette (ABC) transporters. P Glycoprotein is an ATP-dependent drug efflux pump expressed in liver, kidney, small intestine and brain. It is responsible for decreased drug accumulation in multidrug-resistant cells. Genetic variation in P Glycoprotein is thought to play a role in patients who do not respond to drug treatment. P Glycoprotein also functions as a transporter in the blood-brain barrier.

Applications:
Suitable for use in Western Blot and Immunohisochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 0.5-1ug/ml
Immunohistochemistry (Paraffin and Formalin): 1-2ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG10g49Affinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanMouse
Concentration:
Not determined.
Immunogen:
Human and hamster drug-resistant whole cells and crude plasma membranes.
Purity:
Purified by affinity chromatography.
Form
Supplied as a lyophilized powder from 1.2% sodium acetate, 2% BSA, 0.2% sodium azide. Reconstitute with 1ml of PBS.
Specificity:
Recognizes human P Glycoprotein at 141kD.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.