		Technical Data
M2825-14N	MDR1 (Multi Drug Resistance Protein 1, ABC20, ATP Binding Cassette Subfamily B (MDR/TAP) Member 1, ABCB1, CD243, CLCS, GP170, P-Glycoprotein, P-gp, P Glycoprotein 1, PGY1)

Description:
P-glycoprotein, the product of the MDR1 (Multidrug Resistance 1) gene, is expressed in distinct non-malignant cells, typically cells with secretory and excretory functions. It is assumed to function as an ATP-dependent drug efflux pump with broad substrate specificity. The highest expression of the P-glycoprotein has been observed in kidney (proximal tubules), liver (bile canaliculi), adrenal gland and intestine suggesting that the primary role of P-glycoprotein is in the normal secretion of physiological metabolites and ingesting chemicals into bile, urine and the lumen of the intestinal tract. Elevated levels of P-glycoprotein have also been reported in the multidrug-resistant cell lines and in colon, endometrial, ovarian, breast tumors, sarcomas and lymphomas.

Applications:
Suitable for use in Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Immunohistochemistry: Frozen and formalin-fixed, paraffin-embedded tissue sections. Epitope retreival is required using citrate buffer. See protocol.
Optimal dilutions to be determined by the researcher.

Positive Control:
Liver/colon

Expected Staining Pattern:
Membrane

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Type	Isotype	Clone	Grade
Mab	IgG1	6A119	Supernatant

Size		Storage	Shipping	Source	Host
1ml		4°C (-20°C Glycerol)	Blue Ice	Human	Mouse

Concentration:

~0.1mg/ml

Immunogen:

Multi-Drug Resistant Chinese hamster cell line (CHrC5)

Purity:

Tissue culture supernatant

Form

Supplied as a liquid in PBS, pH 7.4, 1% BSA, 0.1% sodium azide.

Specificity:

Recognizes human Multi-Drug Resistance 1.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Juranka, P.F., et al., FASEB J. 3(14): 2583-2592 (1989). 2. Nooter, K., et al., Cytotechnology 12(1-3): 213-230 (1993). 3. Scheper, R.J., et al., Int. J. Cancer 42: 389-394 (1998). 4. Schinkel, A.H., et al., Cancer Res. 51: 2628-2635 (1991). 5. Tiirikainen, M.I., et al., Annals of Hematology 65: 124-130 (1992). 6. Goasquen, J.E., et al., Blood 81: 2394-2398 (1993). 7. Itsubo, M., et al., Cancer 73: 298-303 (1994). 8. Klimecki, W.T., et al., Blood 83: 2451-2458 (1994).