		Technical Data
M2825-15E	MDR1 (Multi Drug Resistance Protein 1, ABC20, ATP Binding Cassette Subfamily B (MDR/TAP) Member 1, ABCB1, CD243, CLCS, GP170, P-Glycoprotein, P-gp, P Glycoprotein 1, PGY1) (FITC)

Description:
MDR1 is a transmembrane protein and member of the ABC transporter (ATP-binding cassette) family, which acts as an active efflux pump for a diverse range of lipophillic compounds.CD243 is expressed at low levels in the cell membrane of peripheral blood leukocytes, and constitutively
expressed on the apical plasma membrane of excretory epithelial cells of the kidney, liver, brain and small intestine. CD243 mediates resistance to many chemotherapeutic agents used for tumor suppression and is therefore of special interest to oncologists. Clone UIC2 is a strong inhibitor of CD243-mediated efflux and of the resistance of MDR cells to CD243 transported cytotoxic drugs.

Applications:
Suitable for use in Flow Cytometry. Other applications not tested.

Recommended Dilutions:
Flow Cytometry: Use 10ul to label 1x10e6 cells in 100ul.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. FITC conjugates are sensitive to light.

Type	Isotype	Clone	Grade
Mab	IgG2a	9A183	Affinity Purified

Size		Storage	Shipping	Source	Host
100ug		-20°C	Blue Ice	Human	Mouse

Concentration:

~0.1mg/ml

Immunogen:

Mouse Balb/c 3T3 fibroblasts transfected with human CD243 cDNA.

Purity:

Purified by Protein G affinity chromatography

Form

Supplied as a liquid in PBS, pH 7.4, 1% BSA, 0.09% sodium azide. Labeled with Fluorescein Isothiocyanate Isomer 1 (FITC).

Specificity:

Recognizes an extracellular conformational epitope of human CD243, also known as MDR1 (multi-drug resistance protein 1) and Pgp (P-glycoprotein). Species Crossreactivity: primate. Does not react with mouse or rat.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Mechetner, E.B and Roninson, I.B. (1992) Efficient inhibition of P-glycoprotein-mediated multidrug resistance with a monoclonal antibody. P.N.A.S. 89: 5824-5828. 2. Park, S.W. et al. (2003) Analysis of P-glycoprotein-mediated membrane transport in human peripheral blood lymphocytes using the UIC2 shift assay.
Cytometry Part A. 53A: 67-78. 3. Koziolek, M.J. et al. (2001) Expression of multidrug resistance P-glycoprotein in kidney allografts from cyclosporine A-treated patients. Kid. Int. 60: 156-166. 4. Beck, W.T. et al. (1996) Methods to detect P-glycoprotein-associated multidrug resistance in patients’ tumours: Consensus recommendations. Cancer. Res. 56: 3010-3020. 5. Meister, S. et al. (2010) Calcium Channel Blocker Verapamil Enhances Endoplasmic Reticulum Stress and Cell Death Induced by Proteasome Inhibition in Myeloma Cells Neoplasia. 12: 550-61.