Technical Data
M2825-18C
MDR3 (MRP3, Multi Drug Resistance Associated Protein, cMOAT2, Canalicular Multi Organic Anion Transporter 2)
Description:
Applications:
Suitable for use in Immunofluorescence, Flow Cytometry, ELISA, Western Blot, Immunohistochemistry and Immunocytochemistry. Other applications not tested.

Recommended Dilution:
Flow Cytometry: Weak, cell permeabilization required.
Western Blot: Use 1:20-1:50 and IgG (HRP) anti-mouse.
Immunocytochemistry: 1:20-1:50 on acetone or formaldehyde fixed cytospin preparations.
Immunohistochemistry (Frozen sections): 1:20 on acetone fixed frozen sections followed by incubation with IgG rabbit anti-mouse and a monoclonal mouse APAAP complex. Alternatively, after incubation with the primary antibody (1:20) and washing, slides can be incubated with IgG (Biotin) rabbit anti-mouse and Streptavidin (HRP).
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG16D568Supernatant
SizeStorageShippingSourceHost
250ug-20CBlue IceHumanMouse
Concentration:
Not determined.
Immunogen:
Recombinant human MRP3 (multidrug resistance-associated protein 3) (aa830-949).
Purity:
Supernatant
Form
Supplied as a liquid, 0.7% BSA, 0.1% sodium azide.
Specificity:
Recognizes an internal epitope of human MRP3. Does not crossreact with human P-glycoprotein, MDR3 P-glycoprotein, MRP1, MRP2 (cMOAT) or MRP5. Does not crossreact with mouse, rat or guinea pig MRP2.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Kool, M., et al., PNAS 96: 6914 (1999). 2. Scheffer, G.L., et al., Cancer Res. 60: 5269 (2000). 3. Scheffer, G.L., et al., Lab. Invest. 82: 193 (2002). 4. Kool, M., et al., Cancer Res. 57: 3537 (1997).