		Technical Data
M2825-18C	MDR3 (MRP3, Multi Drug Resistance Associated Protein, cMOAT2, Canalicular Multi Organic Anion Transporter 2)

Description:
Applications:
Suitable for use in Immunofluorescence, Flow Cytometry, ELISA, Western Blot, Immunohistochemistry and Immunocytochemistry. Other applications not tested.

Recommended Dilution:
Flow Cytometry: Weak, cell permeabilization required.
Western Blot: Use 1:20-1:50 and IgG (HRP) anti-mouse.
Immunocytochemistry: 1:20-1:50 on acetone or formaldehyde fixed cytospin preparations.
Immunohistochemistry (Frozen sections): 1:20 on acetone fixed frozen sections followed by incubation with IgG rabbit anti-mouse and a monoclonal mouse APAAP complex. Alternatively, after incubation with the primary antibody (1:20) and washing, slides can be incubated with IgG (Biotin) rabbit anti-mouse and Streptavidin (HRP).
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Type	Isotype	Clone	Grade
Mab	IgG1	6D568	Supernatant

Size		Storage	Shipping	Source	Host
250ug		-20°C	Blue Ice	Human	Mouse

Concentration:

Not determined.

Immunogen:

Recombinant human MRP3 (multidrug resistance-associated protein 3) (aa830-949).

Purity:

Supernatant

Form

Supplied as a liquid, 0.7% BSA, 0.1% sodium azide.

Specificity:

Recognizes an internal epitope of human MRP3. Does not crossreact with human P-glycoprotein, MDR3 P-glycoprotein, MRP1, MRP2 (cMOAT) or MRP5. Does not crossreact with mouse, rat or guinea pig MRP2.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Kool, M., et al., PNAS 96: 6914 (1999). 2. Scheffer, G.L., et al., Cancer Res. 60: 5269 (2000). 3. Scheffer, G.L., et al., Lab. Invest. 82: 193 (2002). 4. Kool, M., et al., Cancer Res. 57: 3537 (1997).