Technical Data
Media
Medium 199 Modified w/ Hanks’, L-Glutamine (Powder, TC 199)
M2852
Cell Culture Grade
Storage RT/4°C    Shipping RT
Components shown as mg/liter
Inorganic Salts:
Calcium Chloride•2H2O185.50
Ferric Nitrate•9H2O0.72
Potassium Chloride400.00
Potassium Phosphate Monobasic60.00
Magnesium Sulfate97.67
Sodium Chloride8000.00
Sodium Phosphate Dibasic47.88
Amino Acids:
L-Alanine25.00
L-Arginine HCl70.00
L-Aspartic Acid30.00
L-Cysteine•HCI0.0987
L-Cystine•2Na•H2O25.16
L-Glutamic Acid66.82
L-Glutamine100.00
Glycine50.00
L-Histidine•HCl•H2O21.88
L-Hydroxyproline10.00
L-Isoleucine20.00
L-Leucine60.00
L-Lysine•HCI70.00
L-Methionine15.00
.L-Phenylalanine25.00
L-Proline40.00
L-Serine25.00
L-Threonine30.00
L-Tryptophan10.00
L-Tyrosine•2Na•2H2O57.66
L-Valine25.00
Vitamins:
L-Ascorbic Acid0.05
Biotin0.01
D-Pantothenic Acid•Ca0.01
Choline Chloride0.50
Calciferol0.10
Folic Acid0.01
i-Inositol0.05
Menadione•NaHSO3•3H2O0.019
Nicotinamide (Niacinamide)0.025
Niacin (Nicotinic Acid)0.025
P-Aminobenzoic Acid0.05
Pyridoxal•HCI0.025
Pyridoxine•HCI0.025
Riboflavin0.01
Thiamine•HCI0.01
Vitamin A Acetate0.140
DL-Tocopherol PO4•2Na0.010
Other:
Adenine Sulfate•2H2O10.98
ATP•2Na1.00
5AMP•H2O (Adenylic Acid )0.2104
Cholesterol0.20
2-Deoxy-D-ribose0.50
Dextrose (D-Glucose)1000.00
Glutathione0.05
Guanine•HCl0.30
Hypoxanthine0.30
Phenol Red, Sodium17.00
D-Ribose0.50
Sodium Acetate50.00
Thymine0.30
Tween 80®20.00
Uracil0.30
Xanthine0.30
Total:10.671 g/liter
In 1950, Morgan, Morton and Parker described one of the first totally defined media that did not depend largely on animal products or extracts as nutritive sources. This medium supported the growth of primary chick embryo heart cells. It has since become known as Medium 199. Its current use is with the addition of serum in virus and vaccine production, and in the culture of many non-transformed cells. It is also used in combination with less complex media. Medium 199 modified with Hanks’ Balanced Salts is designed for use with cells maintained in ambient (non-CO2) atmospheric conditions. M2852 differs from the original formulation by containing all L-amino acids. The original formulation contained 10 DL-amino acids, which are less biologically active on a per gram basis than the corresponding L-amino acids.


Directions per Liter: Dissolve 10.67g in 800-900ml of ddH2O stirring gently until completely solubilized. Add 0.35g/L sodium bicarbonate. Adjust pH of the medium to the desired level. Add additional water to bring the solution to1L Filter-sterilize using a 0.22 micron membrane filter. Aliquot into sterile containers. Do not autoclave. Contains heat-labile compounds that can be damaged with autoclaving.

Storage and Stability: Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.

Appearance: Light yellow to peach, homogeneous, free flowing powder

Solubility: Yellow to orange, clear, complete

pH: As Reported

Endotoxin: 1EU/ml


Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

1. Morgan, J.F., Morton, H.J., Parker, R.C., Proc. Soc. Exp. Biol. Med. 73: 1-8 (1950). 2. Morgan, J.F., Campbell, E., Morton, H.J., J.N.C.I. 16(2): 557-567 (1955). 3. Rutzky, L.P., Pumper, R.W., In Vitrol 9: 468 (1974). 4. Morton, H.J., Toinai, S., TCA Manual 4: 729 (1978). 5. Ryan, J.M., Walk, P.H., TCA Manual 5: 1043 (1979). 6. Bolton, W.E., Terrell, S.P., et al., Tissue Culture Methods 7: 39 (1982).