Technical Data
Media
Medium 199 Hanks’, w/L-Glutamine, HEPES (Powder, TC 199)
M2853
Cell Culture Grade
Storage RT/4°C    Shipping RT
Components shown as g/liter
Inorganic Salts:
Calcium Chloride•2H2O0.185
Ferric Nitrate•9H2O0.00072
Magnesium Sulfate0.09767
Potassium Chloride0.4
Potassium Phosphate Monobasic0.06
Sodium Acetate0.05
Sodium Chloride8
Sodium Phosphate Dibasic0.04788
Amino Acids:
L-Alanine0.025
L-Arginine•HCl0.07
L-Aspartic Acid0.03
L-Cysteine•HCL•H2O0.00011
L-Cystine•2HCl0.026
L-Glutamic Acid0.0668
L-Glutamine0.1
Glycine0.05
L-Histidine•HCl•H2O0.02188
Hydroxy-L-proline0.01
L-Isoleucine0.02
L-Leucine0.06
L-Lysine•HCl0.07
L-Methionine0.015
L-Phenylalanine0.025
L-Proline0.04
L-Serine0.025
L-Threonine0.03
L-Tryptophan0.01
L-Tyrosine•2Na•2H2O0.05766
L-Valine0.025
Vitamins:
P-Aminobenzoic Acid0.00005
Ascorbic Acid•Na0.0000566
D-Biotin0.00001
Choline Chloride0.0005
Calciferol0.0001
myo-Inositol0.00005
Menadione (sodium bisulfate)0.000016
Nicotinic Acid0.000025
Niacinamide0.000025
D-Pantothenic Acid•Ca0.00001
Pyridoxal•HCl0.000025
Pyridoxine•HCl0.000025
Riboflavin0.00001
Thiamine•HCl0.00001
DL-a-Tocopherol Phosphate•2Na0.00001
Retinol Acetate0.00014
Other:
Adenine Sulfate0.01
Adenosine Monophosphate0.0002385
Adenosine Triphosphate•2Na0.001
Cholesterol0.0002
Deoxyribose0.0005
Thymine0.0003
D-Glucose1
Glutathione0.00005
Guanine•HCl0.0003
HEPES5.958
Hypoxanthine0.0003
Phenol Red (sodium)0.0213
Tween 800.02
Ribose0.0005
Uracil0.0003
Xanthine•Na0.000344
Total:16.63g/L



Directions per Liter: Dissolve 16.75g in 800-900ml of ddH2O stirring gently until completely solubilized. Add 0.35g/L sodium bicarbonate. Adjust pH of the medium to 0.1-0.3 pH unit below the desired level. Add additional water to bring the solution to1L Filter-sterilize using a 0.22 micron membrane filter. Aliquot into sterile containers. Do not autoclave. Contains heat-labile compounds that can be damaged with autoclaving.

Storage and Stability: Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.

Appearance: Light yellow to peach, homogeneous, free flowing powder

Solubility: Yellow to orange, clear, complete

pH: As Reported

Endotoxin: 1EU/ml


Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

1. Morgan, J.F., Morton, H.J. and Parker, R.C. Proc. Soc. Exp. Biol. Med. 73:1-8 (1950). 2. Morgan, J.F., Campbell, E. and Morton, H.J. J.N.C.I. 16:2, 557-567 (1955)