Technical Data
Media
Medium 199 Hanks’, w/L-Glutamine, HEPES (Powder, TC 199)
M2853
Cell Culture Grade
Storage RT    Shipping RT
Components shown as g/liter
Inorganic Salts:
Calcium Chloride•2H2O0.185
Ferric Nitrate•9H2O0.00072
Magnesium Sulfate0.09767
Potassium Chloride0.4
Potassium Phosphate Monobasic0.06
Sodium Acetate0.05
Sodium Chloride8
Sodium Phosphate Dibasic0.04788
Amino Acids:
L-Alanine0.025
L-Arginine•HCl0.07
L-Aspartic Acid0.06
L-Cysteine•HCL•H2O0.00011
L-Cystine•2HCl0.026
L-Glutamic Acid0.1336
L-Glutamine0.1
Glycine0.05
L-Histidine•HCl•H2O0.02188
Hydroxy-L-proline0.01
L-Isoleucine0.04
L-Leucine0.06
L-Lysine•HCl0.07
Hydroxy-L-proline0.01
L-Isoleucine0.04
L-Leucine0.06
L-Lysine•HCl0.07
L-Methionine0.015
L-Phenylalanine0.025
L-Proline0.04
L-Serine0.025
L-Threonine0.03
L-Tryptophan0.01
L-Tyrosine•2Na•2H20.05766
L-Valine0.025
Vitamins:
Ascorbic Acid•Na0.0000566
D-Biotin0.00001
Calciferol0.0001
Choline Chloride0.0005
Cocarboxylase0.00001
Menadione (sodium bisulfate)0.000016
myo-Inositol0.00005
Niacinamide0.000025
NicotinicAcid0.000025
D-Pantothenic Acid•Ca0.00001
P-Aminobenzoic Acid0.00005
Pyridoxal•HCl0.000025
Pyridoxine•HCl0.000025
Retinol Acetate0.00014
Riboflavin0.00001
Thiamine•HCl0.00001
DL-a-Tocopherol Phosphate•2Na0.00001
Other:
AdenineSulfate0.01
Adenosine Triphosphate•2Na0.001
Adenosine Monophosphate0.0002385
Cholesterol0.0002
Deoxyribose0.0005
D-Glucose1
Glutathione0.00005
Guanine•HCl0.0003
HEPES5.958
Hypoxanthine0.0003
Tween 800.02
Phenol Red (sodium)0.0213
Ribose0.0005
Thymine0.0003
Uracil0.0003
Xanthine•Na0.000344
Total:16.75 g/L



Directions per Liter: Dissolve 16.75g in 800-900ml of ddH2O stirring gently until completely solubilized. Adjust pH of the medium to the desired level. Add additional water to bring the solution to1L If required, add sodium bicarbonate with stirring after solution has cooled. Filter-sterilize using a 0.22 micron membrane filter. Aliquot into sterile containers. Do not autoclave. Contains heat-labile compounds that can be damaged with autoclaving.

Storage and Stability: Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.

Appearance: White to light tan, homogeneous and free flowing powder

Solubility: Yellow, clear, complete

pH: 4.5 ± 0.4


Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

1. Morgan, J.F., Morton, H.J. and Parker, R.C. Proc. Soc. Exp. Biol. Med. 73:1-8 (1950)
2. Morgan, J.F., Campbell, E. and Morton, H.J. J.N.C.I. 16:2, 557-567 (1955)