Technical Data
Media
Medium 199 w/ Earle’s w/o L-Glutamine (10X) (Powder, TC 199)
M2857
Cell Culture Grade
Storage RT    Shipping RT
Components shown as mg/liter
Inorganic Salts:
Calcium Chloride2000
Ferric Nitrate•9H2O7.2
Magnesium Sulfate977
Potassium Chloride4000
Sodium Acetate500
Sodium Chloride68000
Sodium Phosphate Monobasic1400
Amino Acids:
L-Alanine250
L-Arginine•HCl700
L-Aspartic Acid300
L-Cysteine•HCL•H2O1.1
L-Cystine•2HCl260
L-Glutamic Acid750
Glycine500
L-Histidine•HCl•H2O218.8
Hydroxy-L-proline100
L-Isoleucine200
L-Leucine600
L-Lysine•HCl700
L-Methionine150
L-Phenylalanine250
L-Proline400
L-Serine250
L-Threonine300
L-Tryptophan100
L-Tyrosine•2Na•2H2O576.6
L-Valine250
Vitamins:
L-Ascorbic Acid0.5
D-Biotin0.1
Calciferol1
Choline Chloride5
Folic Acid0.1
Menadione, Na Bisulfate•3H2O0.19
i-Inositol0.5
Niacinamide0.25
Nicotinic Acid0.25
D-Pantothenic Acid•Ca0.1
P-Aminobenzoic Acid0.5
Pyridoxal•HCl0.25
Pyridoxine•HCl0.25
Vitamin A (Retinol) Acetate1.4
Riboflavin0.1
Thiamine•HCl0.1
DL-a-Tocopherol•PO4•2Na0.1
Adenine Sulfate100
Other:
Adenosine Triphosphate•2Na10
5'-Adenylic acid2
Cholesterol2
2'-Deoxy-D-ribose5
D-Glucose10000
Glutathione0.5
Guanine•HCl3
Hypoxanthine3.5
Tween® 8050
D-Ribose5
Thymine3
Uracil3
Xanthine•Na3.4
Phenol Red, Sodium100
Total94g/liter
In 1950, Morgan, Morton and Parker described one of the first totally defined media that did not depend largely on animal products or extracts as nutritive sources. This medium supported the growth of primary chick embryo heart cells. It has since become known as Medium 199. Its current use is with the addition of serum in virus and vaccine production, and in the culture of many non-transformed cells. It is also used in combination with less complex media. Medium 199 modified with Earle's Balanced Salts is designed for use with cells maintained in ambient (non-CO2) atmospheric conditions. M2852 differs from the original formulation by containing all L-amino acids. The original formulation contained 10 DL-amino acids, which are less biologically active on a per gram basis than the corresponding L-amino acids.

The search for a synthetic medium to replace serum for maintaining cells in vitro began in the late nineteenth century and continues to this day. Ringer, Locks and Tyrode substituted physiological salt solutions augmented with glucose for serum and thereby laid the foundation for the development of defined media. As biochemical and analytical techniques have improved, more of the components in serum such as vitamins, hormones, and amino acids have been identified and incorporated into physiological salt solutions, reducing, and in some cases eliminating the concentrations of animal sera required as a medium supplement. Although there have been many modifications to the original formulas in efforts to produce fully defined media, salt solutions still play an important role in tissue culture. A salt solution's basic function, to maintain the pH and osmotic balance in the medium and to provide the cells with water and essential inorganic ions, is as valuable today as when it was first developed a century ago. Earle’s Balanced Salts selection is strongly influenced by 1. type of cell, 2. type of culture monolayer, suspension, clonal, and 3. degree of chemical definition necessary. It is important to review the literature for recommendations concerning medium, supplementation and physiological parameters required for a specific cell line.

Appearance:
Off-white to slight yellow, homogeneous, free flowing powder

Solubility:
Off-white to slight yellow, clear, complete

pH:
As reported

Endotoxin:
1EU/ml


Directions (10X Solution): 1. Dissolve 94 grams per liter of distilled/deionized water, heating with stirring until completely solubilized. 2. Adjust to desired pH.

3. Filter sterilize Note: Some precipitate may be observed due to excess concentration of salts. Sterile filter after solubilization.USB recommends the preparation of this product as a 1X solution.Precautions:Contains heat-labile compounds that can be damaged with autoclaving. This product should be sterilized with a 0.2µ filter.Makes a 10X Solution


Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

1. Morgan, J.F., Morton, H.J. and Parker, R.C. Proc. Soc. Exp. Biol. Med. 73:1-8 (1950)
2. Morgan, J.F., Campbell, E. and Morton, H.J. J.N.C.I. 16:2, 557-567 (1955)