Technical Data
M2867-61C
MARCH-VI, aa701-712 (TEB4, MARCH6, membrane-associated ring finger (C3HC4) 6, KIAA0597, RNF176, TEB4, membrane-associated RING-CH protein VI)
Description:
Applications:
Suitable for use in ELISA, Western Blot. Other applications not tested.

Recommended Dilution:
Peptide ELISA: 1:16,000.
Western Blot: 0.2-0.5ug/ml. Approx 100kD band observed in Human Lung lysates (calculated MW of 103kD according to NP_005876.2). Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Peptide Blocking:
Corresponding peptide is available for Peptide Blocking studies. See M2867-61C-P. Antibody is typically 0.5mg/ml and peptide is supplied as a 100ul pellet. When peptide is reconstituted in 200ul water, the concentration would also be 0.5mg/ml. To start, the best ratio would be 1:1 (molar excess of peptide relative to antibody when identical volumes are mixed). Mix equal volumes of peptide and antibody at the required dilution and leave at ambient temperature. It is best is to have two identical blots to be incubated with equal amount of antibody, but one with the antibody pre-adsorbed to the peptide for 20min. Then incubate and develop the two blots in parallel.
TypeIsotypeCloneGrade
PabAffinity Purified
SizeStorageShippingSourceHost
100ug-20°CBlue IceHumanGoat
Concentration:
~0.5mg/ml
Immunogen:
A synthetic peptide from the C-terminus of aa--QGRRVIFQKVKE.
Purity:
Purified by affinity chromatography.
Form
Supplied as a liquid in Tris saline, pH 7.2, 0.5% BSA, 0.02% sodium azide.
Specificity:
Recognizes mouse MARCH6.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
Membrane topology of the yeast endoplasmic reticulum-localized ubiquitin ligase Doa10 and comparison with its human ortholog TEB4 (MARCH-VI)., Kreft SG, Wang L, Hochstrasser M., J Biol Chem. 2006 Feb 24;281(8):4646-53. Epub 2005 Dec 22.