Technical Data
M2990-02
Merosin (Laminin alpha 2)
Description:
Laminin, an extracellular protein, is a major component of the basement membrane. It is thought to mediate the attachment, migration, and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components. It is composed of three subunits, alpha, beta, and gamma, which are bound to each other by disulfide bonds into a cross-shaped molecule. The LAMA2 gene encodes the alpha 2 chain, which constitutes one of the subunits of laminin 2 (merosin) and laminin 4 (s-merosin). Mutations in this gene have been identified as the cause of congenital merosin-deficient muscular dystrophy.

Applications:
Suitable for use in ELISA, Immunoprecipitation and Immunohistochemistry. Not recommended for Western Blot. Other applications have not been tested.

Recommended Dilutions:
ELISA: 0.02-0.1ug/ml
Immunoprecipitation: 1-10ug/ml in PBS.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG16D580Affinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceMouseRat
Concentration:
~0.1mg/ml (after reconstitution)
Immunogen:
Mouse heart laminin-2 (merosin).
Purity:
Purified by subsequent thiophilic adsorption and size exclusion chromatography.
Form
Supplied as a lyophilized powder from PBS, 0.09% sodium azide, PEG and sucrose. Reconstitute with 1ml sterile ddH2O (15 minutes, RT).
Specificity:
Recognizes mouse laminin alpha2 at 300kD. Recognizes an N-terminal portion of the alpha2 chain that is deleted in congenital muscular dystrophies. Species Crossreactivity: human
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
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