Technical Data
M3300-05B
5-Methoxytryptamine, Conjugated
Description:
Applications:
Suitable for use in ELISA, Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile dH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 2 months at 4°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG11n206Purified
SizeStorageShippingSourceHost
100ul-20°CBlue IceMouse
Concentration:
Not determined
Immunogen:
Polyclonal or monoclonal conjugated serotonin.
Purity:
Purified by protein carrier (BSA) affinity column, and ammonium sulphate precipitation.
Form
Supplied as a lyophilized powder. Reconstitute with 50ul sterile dH2O.
Specificity:
Recognizes protein-linked 5-Methoxytryptamine. Using an antibody ,specificity was performed with an ELISA test by competition experiments with the following compounds : Anti-conjugated 5-Hydroxytryptamine antibody 1 Anti-conjugated 5-Hhydroxytryptophan antibody 1/20 Anti-conjugated 5-Methoxytryptamine antibody 1/60
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. BONA CA. Parallel sets and the internal image of antigen within the idiotypic network. Fed Proc. 1984 Jul;43(10):2558-62. 2. FIELDS BA, GOLDBAUM FA, YSERN X, POLJAK RJ, MARIUZZA RA. Molecular basis of antigen mimicry by an anti-idiotope. Nature. 1995 20;374(6524): 739-42. 3. SOUZA EB, LOPES JD, ALMEIDA SR. B and T cell responses elicited by monoclonal anti-idiotypic antibody (Ab2beta) mimicking gp43 from Paracoccidioides brasiliensis. Clin Exp Immunol. 2004 Jul;137(1):123-8. 4. PILLET D, PAON M, VOROBIEV II, GABIBOV AG, THOMAS D, FRIBOULET A. Idiotypic network mimicry and antibody catalysis: lessons for the elicitation of efficient anti-idiotypic protease antibodies. J Immunol Methods. 2002 Nov 1;269(1-2):5-12. • JENNINGS I, COTTON R. Structural similarities among enzyme pterin binding sites as demonstrated by a monoclonal anti-idiotypic antibody. J Biol Chem. 1990 Feb5;265(4):1885-9.