Technical Data
M3390-01C
AMACR (Alpha-methylacyl-CoA Racemase, 2-methylacyl-CoA Racemase)
Description:
Alpha-methyacyl-CoA racemase (AMCR), is an enzyme invloved in beta oxidation of branched chain fatty acids and bile salt intermediates, and has recently been identified as a neomarker for prostate cancer, where it is over expressed. Several different isoforms have been reported that are produced either by extensinve alternatve splicing of 5 exons or by use of alternate initiation codons. At least 2 different transcripts, each derived from the 5 exons, have been reported, AMCR I and AMCR II. AMCR I is the most abundant form and enclodes for a 382aa protein (42kD). The other isoform AMCR II exhibits significant homolgy to fumarate hydratase and encodes a 288aa protein (32kD). Several other variants of IA and IIA isoforms have been characterized recently.

Applications:
Suitable for use in Immunofluorescence, Western Blot, ELISA and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Immunofluorescence: 1:200-1:1000
ELISA: 1:10,000
Western Blot: 1:500-1:2000
Immunohistochemistry (Formalin fixed paraffin embedded): 1:200
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG2bAscites
SizeStorageShippingSourceHost
50ul-20CBlue IceHumanMouse
Concentration:
Not determined
Immunogen:
Recombinant corresponding to human AMACR-His fusion protein, expressed in E. coli strain BL21 (DE3)
Purity:
Ascites
Form
Supplied as a liquid.
Specificity:
Recognizes human AMACR.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.