Technical Data
M3398-55M
5-Methylcytidine
Description:
Applications:
Suitable for use in Immunofluorescence, Flow Cytometry, Immunoblot, Methylated DNA Immunoprecipitation, and Immunohistochemistry (frozen and paraffin). Other applications not tested.

Recommended Dilution:
Flow Cytometry: 10ul labels 10e6 cells in 100ul
Immunohistochemistry (paraffin): Requires antigen retrieval using heat treatment prior to staining of paraffin sections.
Optimal dilutions to be determined by the researcher.

Hybridoma:
Sp2/0-Ag 14 myeloma cells with spleen cells from Balb/c mice.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG110B11Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceMouse
Concentration:
~1mg/ml
Immunogen:
5-Methylcytidine.
Purity:
Purified
Form
Supplied as a liquid in PBS, pH 7.4, 0.01% thimerosal.
Specificity:
Recognizes the modified base 5-methylcytidine (5-MeCyd) found in DNA of plants and vertebrates. Species Crossreactivity: human, rat, mouse.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Reynaud, C. et al. (1992) Monitoring of urinary excretion of modified nucleosides in cancer patients using a set of six monoclonal antibodies. Cancer Lett. 61: 255-262. 2. Habib, M. et al. (1999) DNA Global Hypomethylation in EBV - Transformed Interphase Nuclei. Exp. Cell. Res. 249: 46-53. 3. Hernandez-Blazquez, F. et al. (2000) Evaluation of global DNA hypomethylation in human colon cancer tissues by immunohistochemistry and image analysis. Gut. 47: 689-693. 4. Giraldo, A. M. et al. (2007) DNA methylation and histone acetylation patterns in cultured bovine fibroblasts for nuclear transfer. Mol. Reprod. Dev. 74: 1514-1524.