Technical Data
5-Methylcytidine (5-Methyl Cytidine)
5-Methylcytidine is a modified base found in DNA of plants and vertebrates. DNA methylation is a post- replication process involved in the establishment of genomic imprinting, in the control of gene expression and of differentiation. Carcinogenesis is associated with alterations of the DNA methylation pattern: a global DNA hypomethylation is often detected in tumor tissues, associated with local hypermethylation sites. This antibody has been developed to discriminate between the modified base and its normal counterpart. lt has been used to detect alterations in the urinary excretion of nucleosides by cancer patients, to visualize the distribution of methyl-rich regions along human chromosomes, to quantify in situ differences between normal and malignant cells from peripheral blood as well as on tissue sections.

Suitable for use in Immunoprecipitation, Western Blot, Flow Cytometry and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Immunocytochemistry: 1:500
Western Blot: 1:500
Immunohistochemistry: 1:500
Immunoprecipitation: 1:50
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
100ug-20CBlue IceMouse
Supplied as a liquid in PBS, 0.01% thimerosal.
Recognizes 5-Methylcytidine.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Reynaud, C. et al. Anal. Letters 23, 31 (1990). 2. Sasco, A.J. et al. Cancer Letters 108, 157 (1996). 3. Rougier, N. et al. Genes and development 14, 2108 (1998). 4. Habib, M. et al. Exp. Cell Res. 249, 46(1999). 5. Weber M. et al. et al. Nature Genetics 37, 853 (2005).