Technical Data
M3800
Micrococcal Nuclease (S7) S. aureus (Strain Foggi)
15KU
45KU
Molecular Biology Storage: -20CShipping: Blue Ice
Micrococcal nuclease is the extracellular nuclease of S. aureus. It catalyzes preferential endohydrolysis of nucleic acids at adenylate, uridylate, deoxyadenylate or thymidylate-rich sites, yielding 3I nucleotides.

Storage and Stability:
Lyophilized powder may be stored at 4C. Reconstitute to nominal volume (1mg/ml) in dH2O. Reconstituted product may be stored at 4C for short term only. For long term storage, aliquot and store at -20C. Reconstituted product is stable for 6 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

CAS Number:
9013-53-0

Molecular Weight:
16.8kD
Source:
S. aureus (Strain Foggi)

Form:
Supplied as a lyophilized powder.

Purity:
Chromatographically purified. Verified by SDS-PAGE.

% Protein:
~60

Protease:
None Detected

Optimum pH:
9.2. However, the enzymic activity has an absolute requirement for Ca2+ and the pH optimum varies according to Ca2+ concentration.

Extinction Coefficient:
9.2

Activity:
5000 units/mg dry weight. Ca2+ is essential for activity. Both RNase and DNase activities are competitively inhibited by deoxythymidine 3',5' diphosphate. The kinetics of degradation of RNA and DNA differ. However, the enzymic activity has an absolute requirement for Ca2+ and the pH optimum varies according to Ca2+ concentration.

Unit Definition:
Based upon the release of acid soluble oligonucleotides following nuclease digestion of DNA. One unit corresponds to a change in optical density of 1.0 at 260 nm at 37C and pH 8.0 using DNA as the substrate.

Specificity:
Exhibits both exo-and endo-5'-phosphodiesterase activities against both DNA and RNA. The enzyme catalyzes preferential endohydrolysis of the RNA and DNA at sites rich in adenylate or uridylate and deoxyadenylate or thymidylate.

Inhibitors: 5'-dioxynucleotides and 5'-ribonucleotides are inhibitory with the former demonstrating greatest inhibition.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
1. Heins, J., et al., (1967) J. Biol. Chem. 242:1016. 2. Heins, J., et al., (1966) Proc. Nucl. Acid Res. Harper and Rowe. 3. Alexander, M., et al., (1961) J. Biol. Chem. 236:3014.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.