Technical Data
Media
MEM Alpha Modified w/L-Glutamine w/o Ribo & Deoxyribonucleosides (Powder)
M3850
Cell Culture Grade
Storage RT/4°C    Shipping RT
Components shown as g/liter
Inorganic Salts:
Calcium Chloride•2H2O0.265
Magnesium Sulfate0.09767
Potassium Chloride0.4
Sodium Chloride6.8
Sodium Phosphate Monobasic0.122
Amino Acids:
L-Alanine0.025
L-Arginine•HCl0.126
L-Asparagine•H2O0.05
L-Aspartic Acid0.03
L-Cysteine•HCL•H2O0.1
L-Cystine•2HCl0.0313
L-Glutamic Acid0.075
L-Glutamine0.292
Glycine0.05
L-Histidine•HCl•H2O0.042
L-Isoleucine0.052
L-Leucine0.052
L-Lysine•HCl0.0725
L-Methionine0.015
L-Phenylalanine0.032
L-Proline0.04
L-Serine0.025
L-Threonine0.048
L-Tryptophan0.01
L-Tyrosine•2Na•2H2O0.0519
L-Valine 0.046
Vitamins:
L-Ascorbic Acid•Na0.05
D-Biotin0.0001
Choline Chloride0.001
VitaminB-120.00136
Folic Acid0.001
myo-Inositol0.002
Niacinamide0.001
D-Pantothenic Acid•Ca0.001
Pyridoxal•HCl0.001
Riboflavin0.0001
Thiamine•HCl0.001
Other:
D-Glucose1.0
Thioctic Acid0.0002
Phenol Red, Sodium0.011
Pyruvic Acid, Sodium0.11
Total:10.1 g/L
Appearance: Light tan to orange, homogeneous, free flowing powder

Solubility: Yellow to orange, clear, complete

pH: As Reported

Endotoxin: 1EU/ml


Directions per Liter: Dissolve 10.1g in 800-900ml of ddH2O stirring gently until completely solubilized. If required, add sodium bicarbonate. Adjust pH of the medium to the desired level. Add additional water to bring the solution to1L Filter-sterilize using a 0.22 micron membrane filter. Aliquot into sterile containers. Do not autoclave. Contains heat-labile compounds that can be damaged with autoclaving.

Storage and Stability: Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.


Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

1. Stanners, et al., Nature New Biology. 230, 52-54. (1971). 2. Stanners, C.P. and Goldberg, V. J. J. Gen. Virol. 29, 281-296. (1975)