Technical Data
Media
MEM Alpha Modified Ca Free w/L-Glutamine, Na Pantothenate w/o Ribo, Deoxyribosides (Powder)
M3850-01
Cell Culture Grade
Storage RT/4°C    Shipping RT
Components shown as mg/liter
Inorganic Salts:
Magnesium Sulfate97.67
Potassium Chloride400
Sodium Chloride6800
Sodium Phosphate Monobasic121.74
Amino Acids:
L-Alanine25
L-Arginine•HCl126.98
L-Asparagine•H2O50
L-Aspartic Acid30
L-Cysteine•HCI•H2O100
L-Cystine•2HCI31.29
L-Glutamic Acid75
L-Glutamine292
Glycine50
L-Histidine•HCl•H2O42
L-Isoleucine52.4
L-Leucine52.4
L-Lysine•HCI72.46
L-Methionine15
L-Phenylalanine32
L-Proline40
L-Serine25
L-Threonine48
L-Tryptophan10
L-Tyrosine•2Na•2H2051.9
L-Valine46
Vitamins:
Ascorbic Acid50
Biotin0.1
Choline Chloride1
Vitamin B121.36
Folic Acid1
i-Inositol2
Nicotinamide1
D-Na Pantothenate1
Pyridoxal HCI1
Riboflavin0.1
Thiamine HCI1
Other Components:
D-Glucose1000
Lipoic Acid0.2
Phenol Red10.2
Sodium Pyruvate110
Total:9.9g/liter



Directions per Liter: Dissolve 9.9g in 800-900ml of ddH2O stirring gently until completely solubilized. If required, add sodium bicarbonate. Adjust pH of the medium to the desired level. Add additional water to bring the solution to1L Filter-sterilize using a 0.22 micron membrane filter. Aliquot into sterile containers. Do not autoclave. Contains heat-labile compounds that can be damaged with autoclaving.

Storage and Stability: Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.

Appearance: Light tan to orange, homogeneous, free flowing powder

Solubility (9.9g/L): Yellow to orange, clear, complete

pH: As Reported


Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

1. Stanners, et al., Nature New Biology 230:52-54 (1971) 2. Stanners, C.P. and Goldberg, V. J., J. Gen. Virol. 29:281-296 (1975)