Technical Data

Media

MEM Alpha Modified Ca Free w/L-Glutamine, Na Pantothenate w/o Ribo, Deoxyribosides (Powder)

M3850-01

Cell Culture Grade

Storage RT/4°C Shipping RT

Components shown as mg/liter


Inorganic Salts:
Magnesium Sulfate	97.67
Potassium Chloride	400
Sodium Chloride	6800
Sodium Phosphate Monobasic	121.74

Amino Acids:
L-Alanine	25
L-Arginine•HCl	126.98
L-Asparagine•H2O	50
L-Aspartic Acid	30
L-Cysteine•HCI•H2O	100
L-Cystine•2HCI	31.29
L-Glutamic Acid	75
L-Glutamine	292
Glycine	50
L-Histidine•HCl•H2O	42
L-Isoleucine	52.4
L-Leucine	52.4
L-Lysine•HCI	72.46
L-Methionine	15
L-Phenylalanine	32
L-Proline	40
L-Serine	25
L-Threonine	48
L-Tryptophan	10
L-Tyrosine•2Na•2H20	51.9
L-Valine	46

Vitamins:
Ascorbic Acid	50
Biotin	0.1
Choline Chloride	1
Vitamin B12	1.36
Folic Acid	1
i-Inositol	2
Nicotinamide	1
D-Na Pantothenate	1
Pyridoxal HCI	1
Riboflavin	0.1
Thiamine HCI	1

Other Components:
D-Glucose	1000
Lipoic Acid	0.2
Phenol Red	10.2
Sodium Pyruvate	110

Total:	9.9g/liter

Directions per Liter: Dissolve 9.9g in 800-900ml of ddH2O stirring gently until completely solubilized. If required, add sodium bicarbonate. Adjust pH of the medium to the desired level. Add additional water to bring the solution to1L Filter-sterilize using a 0.22 micron membrane filter. Aliquot into sterile containers. Do not autoclave. Contains heat-labile compounds that can be damaged with autoclaving.

Storage and Stability: Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.

Appearance: Light tan to orange, homogeneous, free flowing powder

Solubility (9.9g/L): Yellow to orange, clear, complete

pH: As Reported

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

1. Stanners, et al., Nature New Biology 230:52-54 (1971) 2. Stanners, C.P. and Goldberg, V. J., J. Gen. Virol. 29:281-296 (1975)