Technical Data

Media

MEM Alpha Modified w/L-Glutamine w/o Ribo & Deoxyribonucleosides, Proline, Serine (Powder)

M3850-10

Cell Culture Grade

Storage RT Shipping RT

Components shown as g/liter


Inorganic Salts:
Calcium Chloride•2H2O	0.265
Magnesium Sulfate	0.09767
Potassium Chloride	0.4
Sodium Chloride	6.8
Sodium Phosphate Monobasic	0.122

Amino Acids:
L-Alanine	0.025
L-Arginine•HCl	0.126
L-Asparagine•H2O	0.05
L-Aspartic Acid	0.03
L-Cysteine•HCL•H2O	0.1
L-Cystine•2HCl	0.0313
L-Glutamic Acid	0.075
L-Glutamine	0.292
Glycine	0.05
L-Histidine•HCl•H2O	0.042
L-Isoleucine	0.052
L-Leucine	0.052
L-Lysine•HCl	0.0725
L-Methionine	0.015
L-Phenylalanine	0.032
L-Threonine	0.048
L-Tryptophan	0.01
L-Tyrosine•2Na•2H2O	0.0519
L-Valine	0.046

Vitamins:
L-Ascorbic Acid•Na	0.05
D-Biotin	0.0001
Choline Chloride	0.001
Folic Acid	0.001
myo-Inositol	0.002
Niacinamide	0.001
D-Pantothenic Acid,•Ca	0.001
Pyridoxal•HCl	0.001
Riboflavin	0.0001
Thiamine•HCl	0.001
VitaminB-12	0.00136

Other:
D-Glucose	1
Pyruvic Acid Na	0.11
Phenol Red Na	0.011
Thioctic Acid	0.0002

Total:	10.1 g/liter

Directions per Liter: 1. Add 900ml distilled/deionized water (25ºC) to a 1 liter container.2. Weigh 10.1g into container, stirring gently until completely solubilized. Heat gently with stirring.

3. Add 2.0g/Liter NaHCO3 with stirring. 4. Adjust pH to ± 0.1-0.3 pH units with 1N HCl or 1N NaOH.5. q.s. to final 1 Liter volume.6. Sterilize with 0.2µ membrane filter. Do not autoclave since heat-labile vitamins will lose their activity.7. Aliquot into sterile containers, store at 0-5ºC. Keep away from light.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Stanners, et al., Nature New Biology. 230, 52-54. (1971)
Stanners, C.P. and Goldberg, V. J. J. Gen. Virol. 29, 281-296. (1975)