Technical Data
Media
MEM Alpha Modified w/L-Glutamine w/o Ribo & Deoxyribonucleosides, Proline, Serine (Powder)
M3850-10
Cell Culture Grade
Storage RT    Shipping RT
Components shown as g/liter
Inorganic Salts:
Calcium Chloride•2H2O0.265
Magnesium Sulfate0.09767
Potassium Chloride0.4
Sodium Chloride6.8
Sodium Phosphate Monobasic0.122
Amino Acids:
L-Alanine0.025
L-Arginine•HCl0.126
L-Asparagine•H2O0.05
L-Aspartic Acid0.03
L-Cysteine•HCL•H2O0.1
L-Cystine•2HCl0.0313
L-Glutamic Acid0.075
L-Glutamine0.292
Glycine0.05
L-Histidine•HCl•H2O0.042
L-Isoleucine0.052
L-Leucine0.052
L-Lysine•HCl0.0725
L-Methionine0.015
L-Phenylalanine0.032
L-Threonine0.048
L-Tryptophan0.01
L-Tyrosine•2Na•2H2O0.0519
L-Valine0.046
Vitamins:
L-Ascorbic Acid•Na0.05
D-Biotin0.0001
Choline Chloride0.001
Folic Acid0.001
myo-Inositol0.002
Niacinamide0.001
D-Pantothenic Acid,•Ca0.001
Pyridoxal•HCl0.001
Riboflavin0.0001
Thiamine•HCl0.001
VitaminB-120.00136
Other:
D-Glucose1
Pyruvic Acid Na0.11
Phenol Red Na0.011
Thioctic Acid0.0002
Total:10.1 g/liter



Directions per Liter: 1. Add 900ml distilled/deionized water (25ºC) to a 1 liter container.2. Weigh 10.1g into container, stirring gently until completely solubilized. Heat gently with stirring.

3. Add 2.0g/Liter NaHCO3 with stirring. 4. Adjust pH to ± 0.1-0.3 pH units with 1N HCl or 1N NaOH.5. q.s. to final 1 Liter volume.6. Sterilize with 0.2µ membrane filter. Do not autoclave since heat-labile vitamins will lose their activity.7. Aliquot into sterile containers, store at 0-5ºC. Keep away from light.


Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Stanners, et al., Nature New Biology. 230, 52-54. (1971)
Stanners, C.P. and Goldberg, V. J. J. Gen. Virol. 29, 281-296. (1975)