Technical Data
Media
MEM Alpha Modified w/L-Glutamine, HEPES w/o Ribo, Deoxyribonucleosides (Powder)
M3851
Cell Culture Grade
Storage RT    Shipping RT
Components shown as mg/liter
Inorganic Salts:
Calcium Chloride264.92
Potassium Chloride400
Magnesium Sulfate97.67
Sodium Chloride6800
Sodium Phosphate Monobasic121.74
Amino Acids:
L-Alanine25
L-Arginine•HCl126.98
L-Asparagine50
L-Aspartic Acid30
L-Cysteine•HC100
L-Cystine•2HCI31.29
L-Glutamic Acid75
L-Glutamine292
Glycine50
L-Histidine•HCl42
L-Isoleucine52.4
L-Leucine52.4
L-Lysine•HCI72.46
L-Methionine15
L-Phenylalanine32
L-Proline40
L-Serine25
L-Threonine48
L-Tryptophan10
L-Tyrosine•2Na51.9
L-Valine46
Vitamins:
Ascorbic Acid50
Biotin0.1
D-Pantothenic Acid•Ca1
Choline Chloride1
Folic Acid1
i-Inositol2
Nicotinamide1
Pyridoxal•HCI1
Riboflavin0.1
Thiamine•HCI1
Vitamin B121.36
Other
D-Glucose1000
HEPES Free Acid 5958
Lipoic Acid0.2
Phenol Red10.2
Sodium Pyruvate110
Total:16.1g/liter



Directions per Liter: 1. Add 900ml distilled/deionized water (25ºC) to a 1 liter container.2. Weigh 16.09g into container, stirring gently until completely solubilized. Heat gently with stirring.

3. Add 2.0g/Liter NaHCO3 with stirring. 4. Adjust pH to ± 0.1-0.3 pH units with 1N HCl or 1N NaOH.5. q.s. to final 1 Liter volume.6. Sterilize with 0.2µ membrane filter. Do not autoclave since heat-labile vitamins will lose their activity.7. Aliquot into sterile containers, store at 0-5ºC. Keep away from light.


Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

1. Eagle, H. et al., J. Biol. Chem. 214:845-847 (1956) 2. Eagle, H. Tissue Culture Association Manual 3:517-520 (1976) 3. Eagle, H. Science 130:432-437 (1959). 4. Eagle, H. Science 122:501 (1955) 5. Stanners, C.P., Eliceiri, G.L. and Green, H. Nature New Biology 230:52-54 (1971) 6. Stanners, C.P. and Goldberg, V. J., J. Gen. Virol. 29:81-296. (1975)