Technical Data
Media
MEM Eagle Modified w/o L-Glutamine (10X) (Powder)
M3855-03
Cell Culture Grade
Storage RT    Shipping RT
Components shown as mg/liter
Inorganic Salts:
Calcium Chloride2000
Magnesium Sulfate970
Potassium Chloride4000
Sodium Chloride68000
Sodium Phosphate Mono•H2O1400
Amino Acids:
L-Arginine•HCl1264
L-Cystine•2HCl312
L-Histidine•HCl•H2O419
L-Isoleucine525
L-Leucine525
L-Lysine•HCl725
L-Methionine149
L-Phenylalanine325
L-Threonine476.4
L-Tryptophan100
L-Tyrosine•Na2•2H2O519
L-Valine469
Vitamins:
Choline Chloride10
Folic Acid10
i-Inositol20
Nicotinamide10
D-Pantothenic Acid•Ca10
Pyridoxal•HCl10
Riboflavin1
Thiamine•HCl10
Other:
D-Glucose10000
Phenol Red, Sodium100
Total:92.4g/L
Minimum Essential Medium (MEM), developed by Harry Eagle, is one of the most widely used of all synthetic cell culture media.  Early attempts to cultivate normal mammalian fibroblasts and certain subtypes of HeLa cells revealed that they had specific nutritional requirements that could not be met by Eagle's Basal Medium (BME). Subsequent studies using these and other cells in culture indicated that additions to BME could be made to aid growth of a wider variety of fastidious cells. MEM, which incorporates these modifications, includes higher concentrations of amino acids so that the medium more closely approximates the protein composition of mammalian cells. MEM has been used for cultivation of a wide variety of cells grown in monolayers.  Optional supplementation of non-essential amino acids to the formulations that incorporate either Hanks' or Eagles' salts has broadened the usefulness of this medium.


Directions per Liter: Dissolve 101.8 grams per liter of distilled/deionized (DDI) water (final manufacturing formulation for 10X), heating with stirring until completely solubilized, adjusting pH as necessary. If required, add 2g/liter sodium bicarbonate with stirring. Sterilize with 0.2um membrane filter. Aliquot into sterile containers.

Note: Some precipitate may be observed due to excess concentration of salts. Sterile filter after solubilization. USB recommends the preparation of this product as a 1X solution.Storage:Store at 0-5ºC (store unopened media at 2-30°C). Opened bottles should be capped tightly and kept in a dark, low humidity environment.

Solubility: Some precipitate may be observed due to excess concentration of salts. Sterile filter after solubilization.

Endotoxin: ~10EU/mg


Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

1. Eagle, H., Tissue Culture Association Manual 3: 517-520 (1976). 2. Eagle, H. Science 122: 501 (1955).