Technical Data
Media
MEM Eagle w/o L-Glutamine, Folic Acid, Phenol Red (Powder)
M3855-06
Cell Culture Grade
Storage RT    Shipping RT
Components: mg/liter
InorganicSalts:
Calcium Chloride•2H2O264.90
Magnesium Sulfate97.70
Potassium Chloride400.00
Sodium Chloride 6800.00
Sodium Phosphate Monobasic•H2O140.30
Amino Acids:
L-Arginine•HCl126.40
L-Cystine24.02
L-GlutamineAbsent
L-Histidine•HCl•H2O42
L-Isoleucine52
L-Leucine52
L-Lysine•HCl72.5
L-Methionine15
L-Phenylalanine32
L-Threonine48
L-Tryptophan10
L-Tyrosine36
L-Valine46
Vitamins:
Choline Chloride1.00
Folic AcidAbsent
i-Inositol2.00
Nicotinamide1.00
D-Pantothenic Acid•Ca1.00
Pyridoxal•HCl1.00
Riboflavin0.10
Thiamine•HCl1.00
Other:
D-Glucose1000.00
Phenol Red, SodiumAbsent
Total:9.266g/L
Minimum Essential Medium (MEM), developed by Harry Eagle, is one of the most widely used of all synthetic cell culture media. Early attempts to cultivate normal mammalian fibroblasts and certain subtypes of HeLa cells revealed that they had specific nutritional requirements that could not be met by Eagle's Basal Medium (BME). Subsequent studies using these and other cells in culture indicated that additions to BME could be made to aid growth of a wider variety of fastidious cells. MEM, which incorporates these modifications, includes higher concentrations of amino acids so that the medium more closely approximates the protein composition of mammalian cells. MEM has been used for cultivation of a wide variety of cells grown in monolayers.  Optional supplementation of non-essential amino acids to the formulations that incorporate either Hanks' or Eagles' salts has broadened the usefulness of this medium.


Directions per Liter Dissolve 9.27g in 800-900ml of ddH2O, stirring gently until completely solubilized. If required, add 2.2g sodium bicarbonate with stirring. Adjust pH of the medium to 0.1-0.3 pH unit below the desired level. Add additional water to bring the solution to 1L. Filter-sterilize using a 0.22 micron membrane filter. Aliquot into sterile containers. Do not autoclave. Contains heat-labile compounds that can be damaged with autoclaving.

Storage and Stability: Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept refrigerated and used within a short period of time.

Appearance: White to light yellow, free flowing, homogeneous powder Solubility


Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

1. Eagle, H., Tissue Culture Association Manual 3: 517-520 (1976). 2. Eagle, H. Science 122: 501 (1955).