Technical Data
Media
MEM Eagle w/Earle’s, Non-essential Amino Acids (0.85g/L), Penicillin, Streptomycin, w/o L-Glutamine (Powder)
M3857-05
Cell Culture Grade
Storage RT    Shipping RT
Components shown in mg/L
Inorganic Salts:
CalciumChloride200
MagnesiumSulfate97.7
PotassiumChloride400
SodiumChloride6800
Sodium Phosphate Monobasic•H2O 140
Amino Acids:
L-Alanine8.9
L-Arginine•HCl126.4
L-Asparagine•H2O15
L-AsparticAcid13.3
L-Cystine•2HCl31.2
L-GlutamicAcid14.7
Glycine7.5
L-Histidine•HCl•H2O41.9
L-Isoleucine52.5
L-Leucine52.5
L-Lysine•HCl72.5
L-Methionine15
L-Phenylalanine32.5
L-Proline11.5
L-Serine10.5
L-Threonine47.64
L-Tryptophan10
L-Tyrosine•2Na•2H2O51.9
L-Valine46.9
Vitamins:
CholineChloride1
FolicAcid1
i-Inositol2
Nicotinamide1
D-PantothenicAcid•Ca1
Pyridoxal•HCl1
Riboflavin0.1
Thiamine•HCl1
Other:
D-Glucose1000
PhenolRed,Sodium10
Penicillin G, Potassium200,000u (~126.18mg)
DihydrostreptomycinSulfate100mg
Total:9.5g/liter
Minimum Essential Medium (MEM), developed by Harry Eagle, is one of the most widely used of all synthetic cell culture media.  Early attempts to cultivate normal mammalian fibroblasts and certain subtypes of HeLa cells revealed that they had specific nutritional requirements that could not be met by Eagle's Basal Medium (BME). Subsequent studies using these and other cells in culture indicated that additions to BME could be made to aid growth of a wider variety of fastidious cells. MEM, which incorporates these modifications, includes higher concentrations of amino acids so that the medium more closely approximates the protein composition of mammalian cells. MEM has been used for cultivation of a wide variety of cells grown in monolayers.  Optional supplementation of non-essential amino acids to the formulations that incorporate either Hanks' or Eagles' salts has broadened the usefulness of this medium.


Directions per Liter: 1. Add 900ml distilled/deionized water (25ºC) to a 1 liter container.2. Weigh 9.5g into container, stirring gently until completely solubilized. Heat gently with stirring.

3. Add 2.0g/Liter NaHCO3 with stirring. 4. Adjust pH to ± 0.1-0.3 pH units with 1N HCl or 1N NaOH.5. q.s. to final 1 Liter volume.6. Sterilize with 0.2µ membrane filter. Do not autoclave since heat-labile vitamins will lose their activity.7. Aliquot into sterile containers, store at 0-5ºC. Keep away from light.


Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

1. Eagle, H., Tissue Culture Association Manual 3:517-520 (1976)
2. Eagle, H. Science 122:501 (1955)