Technical Data
Media
MEM Eagle w/Earle’s, L-Glutamine, Non-essential Amino Acids, HEPES (Powder)
M3858
Cell Culture Grade
Storage RT    Shipping RT
Components shown as mg/liter
Inorganic Salts:
Calcium Chloride264.92
Potassium Chloride400
Magnesium Sulfate97.67
Sodium Chloride6800
Sodium Phosphate Monobasic121.74
Amino Acids:
L-Alanine8.9
L-Arginine•HCl126.98
L-Asparagine15
L-Aspartic Acid13.3
L-Cystine•2HCI31.29
L-Glutamic Acid14.7
L-Glutamine292
Glycine7.5
L-Histidine•HCl42
L-Isoleucine52.4
L-Leucine52.4
L-Lysine•HCI72.46
L-Methionine15
L-Phenylalanine32
L-Proline11.5
L-Serine10.5
L-Threonine48
L-Tryptophan10
L-Tyrosine•2Na51.9
L-Valine46
Vitamins:
D-Pantothenic Acid•Ca1
Choline Chloride1
Folic Acid1
i-Inositol2
Nicotinamide1
Pyridoxal•HCI1
Riboflavin0.1
Thiamine•HCI1
Other:
D-Glucose1000
HEPES Free Acid5958
Phenol Red10.2
Total:15.6g/liter
Minimum Essential Medium (MEM), developed by Harry Eagle, is one of the most widely used of all synthetic cell culture media.  Early attempts to cultivate normal mammalian fibroblasts and certain subtypes of HeLa cells revealed that they had specific nutritional requirements that could not be met by Eagle's Basal Medium (BME). Subsequent studies using these and other cells in culture indicated that additions to BME could be made to aid growth of a wider variety of fastidious cells. MEM, which incorporates these modifications, includes higher concentrations of amino acids so that the medium more closely approximates the protein composition of mammalian cells. MEM has been used for cultivation of a wide variety of cells grown in monolayers.  Optional supplementation of non-essential amino acids to the formulations that incorporate either Hanks' or Eagles' salts has broadened the usefulness of this medium.


Directions per Liter: 1. Add 900ml distilled/deionized water (25ºC) to a 1 liter container.2. Weigh 15.62g into container, stirring gently until completely solubilized. Heat gently with stirring.

3. Add 2.0g/Liter NaHCO3 with stirring. 4. Adjust pH to ± 0.1-0.3 pH units with 1N HCl or 1N NaOH.5. q.s. to final 1 Liter volume.6. Sterilize with 0.2µ membrane filter. Do not autoclave since heat-labile vitamins will lose their activity.7. Aliquot into sterile containers, store at 0-5ºC. Keep away from light.


Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

1. Eagle, H. et al., J. Biol. Chem. 214:845-847 (1956) 2. Eagle, H. Tissue Culture Association Manual 3:517-520 (1976) 3. Eagle, H. Science 130:432-437 (1959). 4. Eagle, H. Science 122:501 (1955) 5. Stanners, C.P., Eliceiri, G.L. and Green, H. Nature New Biology 230:52-54 (1971) 6. Stanners, C.P. and Goldberg, V. J., J. Gen. Virol. 29:81-296. (1975)