Technical Data
Media
MEM Eagle w/Earle’s, L-Glutamine (Powder)
M3859
Cell Culture Grade
Storage RT/4°C    Shipping RT
Components shown as g/liter
Inorganic Salts:
Calcium Chloride•2H2O0.265
Magnesium Sulfate0.09767
Potassium Chloride0.4
Sodium Chloride8.0
Sodium Phosphate Dibasic0.04788
Amino Acids:
L-Arginine•HCl0.126
L-Cystine•2HCl0.0313
L-Glutamine0.292
L-Histidine•HCl•H2O0.042
L-Isoleucine0.052
L-Leucine0.052
L-Lysine•HCl0.0725
L-Methionine0.015
L-Phenylalanine0.032
L-Threonine0.048
L-Tryptophan0.01
L-Tyrosine•2Na•2H2O0.0519
L-Valine0.046
Vitamins:
Choline Chloride0.001
Folic Acid0.001
myo-Inositol0.002
Niacinamide0.001
D-Pantothenic Acid•Ca0.001
Pyridoxal•HCl0.001
Riboflavin0.0001
Thiamine•HCl0.001
Other:
D-Glucose1
Phenol Red, Sodium0.011
Total:10.7g/liter
Minimum Essential Medium (MEM was, developed by Harry Eagle. It is one of the most widely used of all synthetic cell culture media. Early attempts to cultivate normal mammalian fibroblasts and certain subtypes of HeLa cells revealed that these types of cells had specific nutritional requirements that could not be met by Eagle's Basal Medium (BME). Studies using these and other cells in culture indicated that additions to BME could be made to aid growth of a wider variety of fastidious cells. MEM incorporates these modifications which includes higher concentrations of amino acids so that the medium more closely approximates the protein composition of mammalian cells. MEM has been used for cultivation of a wide variety of cells grown in monolayers. Optional supplementation of non-essential amino acids to the formulations that incorporate either Hanks' or Eagles' salts has broadened the usefulness of this medium.


Directions per Liter: Dissolve 10.7g in 800-900ml of ddH2O stirring gently until completely solubilized. If required, add 2.2g/L sodium bicarbonate. Adjust pH of the medium to the desired level. Add additional water to bring the solution to1L Filter-sterilize using a 0.22 micron membrane filter. Aliquot into sterile containers. Do not autoclave. Contains heat-labile compounds that can be damaged with autoclaving.

Storage and Stability: Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.

Appearance: Peach, homogeneous, free-flowing powder

Solubility (10.7g/L): Magenta to red, clear, complete

pH: 8.0±0.4

Endotoxin: 1EU/ml


Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

1. Eagle, H., Tissue Culture Association Manual 3:517-520 (1976). 2. Eagle, H. Science 122:501 (1955)