Technical Data
Media
MEM Eagle w/Earle’s, L-Glutamine w/o Sodium Succinate, Choline Bitartrate (Powder)
M3861-05
Cell Culture Grade
Storage RT    Shipping RT
Components shown as g/liter
Inorganic Salts:
Calcium Chloride•2H2O0.265
MagnesiumSulfate0.09767
Potassium Chloride0.4
SodiumChloride6.8
Sodium Phosphate Monobasic0.14
SuccinicAcid0.075
Amino Acids:
L-Arginine•HCl0.126
L-Cystine•2HCl0.031
L-Histidine•HCl•H2O0.042
L-Isoleucine0.0525
L-Leucine0.0525
L-Lysine•HCl0.0725
L-Methionine0.015
L-Phenylalanine0.0325
L-Threonine0.0476
L-Tryptophan0.01
L-Tyrosine•2Na•2H2O0.0519
L-Valine0.0468
Vitamins:
FolicAcid0.001
myo-Inositol0.002
Niacinamide0.001
D-Pantothenic Acid•Ca0.001
Pyridoxal•HCl0.001
Riboflavin0.0001
Thiamine•HCl0.001
Other:
D-Glucose1
PhenolRed•Na0.0064
Total:9.37g/liter
Minimum Essential Medium (MEM), developed by Harry Eagle, is one of the most widely used of all synthetic cell culture media.  Early attempts to cultivate normal mammalian fibroblasts and certain subtypes of HeLa cells revealed that they had specific nutritional requirements that could not be met by Eagle's Basal Medium (BME). Subsequent studies using these and other cells in culture indicated that additions to BME could be made to aid growth of a wider variety of fastidious cells. MEM, which incorporates these modifications, includes higher concentrations of amino acids so that the medium more closely approximates the protein composition of mammalian cells. MEM has been used for cultivation of a wide variety of cells grown in monolayers.  Optional supplementation of non-essential amino acids to the formulations that incorporate either Hanks' or Eagles' salts has broadened the usefulness of this medium.


Directions per Liter Dissolve 9.37 grams per liter of distilled/deionized (DDI) water. Heat with stirring until completely solubilized. Adjust pH as necessary. If required, add sodium bicarbonate with stirring when solution has cooled. Sterilize with 0.2um membrane filter. Aliquot into sterile containers. Do not autoclave. Contains heat-labile compounds that can be damaged with autoclaving.

Storage Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.

Appearance: Homogenous, free flowing powder Solubility


Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

1. Eagle, H., et al., J. Biol. Chem. 214: 845-847 (1956). 2. Eagle, H., Tissue Culture Association Manual 3: 517-520 (1976). 3. Eagle, H., Science 130: 432-437 (1959). 4. Eagle, H., Science 122: 501 (1955).