Technical Data
Media
MEM Eagle w/ Hanks’, L-Glutamine, Non-essential Amino Acids, HEPES (Powder)
M3862
Storage RT    Shipping RT
Components shown as g/liter
Inorganic Salts:
Calcium Chloride0.1396
Magnesium Sulfate0.09767
Potassium Chloride0.4
Potassium Phosphate Monobasic0.06
Sodium Chloride8
Sodium Phosphate Dibasic0.04788
Amino Acids:
L-Alanine0.0089
L-Arginine•HCl0.126
L-Aspartic Acid0.0133
L-Asparagine•H2O0.015
L-Cystine•2HCl0.0313
L-Glutamic Acid0.0147
L-Glutamine0.292
Glycine0.0075
L-Histidine•HCl•H2O0.042
L-Isoleucine0.052
L-Leucine0.052
L-Lysine•HCl0.0725
L-Methionine0.015
L-Phenylalanine0.032
L-Proline0.0115
L-Serine0.0105
L-Threonine0.048
L-Tryptophan0.01
L-Tyrosine•2Na•2H2O0.0519
L-Valine0.046
Vitamins:
Choline Chloride0.001
Folic Acid0.001
myo-Inositol0.002
Niacinamide0.001
D-Pantothenic Acid•Ca0.001
Pyridoxal•HCl0.001
Riboflavin0.0001
Thiamine•HCl0.001
Other:
D-Glucose1
HEPES Free Acid5.958
Phenol Red, Sodium0.011
Total:16.7g/liter
Minimum Essential Medium (MEM), developed by Harry Eagle, is one of the most widely used of all synthetic cell culture media.  Early attempts to cultivate normal mammalian fibroblasts and certain subtypes of HeLa cells revealed that they had specific nutritional requirements that could not be met by Eagle's Basal Medium (BME). Subsequent studies using these and other cells in culture indicated that additions to BME could be made to aid growth of a wider variety of fastidious cells. MEM, which incorporates these modifications, includes higher concentrations of amino acids so that the medium more closely approximates the protein composition of mammalian cells. MEM has been used for cultivation of a wide variety of cells grown in monolayers.  Optional supplementation of non-essential amino acids to the formulations that incorporate either Hanks' or Eagles' salts has broadened the usefulness of this medium.

Applications:
Light orange, homogenous, free flowing

Solubility:
Yellow, clear, complete

pH:
As reported

Endotoxin:
10Eu/ml


Dissolve 16.7 grams in 900 mL of distilled/deionized (DDI) water, stirring gently until completely solubilized. Do NOT heat. Add 0.35 gram sodium bicarbonate with stirring. Adjust pH of the medium to 0.1-0.3 pH units below the desired pH. Add additional water to bring the solution to final volume. Filter-sterilize using a 0.22 micron membrane filter. Aliquot into sterile containers. Storage:

Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.


Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

1. Eagle, H., Tissue Culture Association Manual 3:517-520 (1976) 2. Eagle, H. Science 122:501 (1955)