Technical Data
Media
MEM Eagle w/ Hanks’, HEPES w/o L-Glutamine, L-Alanine (Powder)
M3863-05
Cell Culture Grade
Storage RT    Shipping RT
Components shown as mg/liter
Inorganic Salts:
Calcium Chloride•2H2O185.5
Potassium Chloride400
Potassium Phosphate Mono60
Magnesium Sulfate •7H2O200
Sodium Chloride8000
Sodium Phosphate Dibasic47.5
Amino Acids:
L-Arginine•HCl126.4
L-Cystine•Na2•H2O30.22
L-Histidine•HCl•H2O41.9
L-Isoleucine52.5
L-Leucine52.5
L-Lysine•HCI73.1
L-Methionine14.9
L-Phenylalanine33.02
L-Threonine47.64
L-Tryptophan10.2
L-Tyrosine•2Na•2H2051.9
L-Valine46.9
Vitamins:
D-Pantothenic Acid•Ca1
Choline Chloride1
Folic Acid1
i-Inositol2
Nicotinamide1
Pyridoxal•HCI1
Riboflavin0.1
Thiamine•HCI1
Other:
D-Glucose1000
HEPES Free Acid4770
Phenol Red, Sodium10
Total:15.3g/liter
Minimum Essential Medium (MEM), developed by Harry Eagle, is one of the most widely used of all synthetic cell culture media.  Early attempts to cultivate normal mammalian fibroblasts and certain subtypes of HeLa cells revealed that they had specific nutritional requirements that could not be met by Eagle's Basal Medium (BME). Subsequent studies using these and other cells in culture indicated that additions to BME could be made to aid growth of a wider variety of fastidious cells. MEM, which incorporates these modifications, includes higher concentrations of amino acids so that the medium more closely approximates the protein composition of mammalian cells. MEM has been used for cultivation of a wide variety of cells grown in monolayers.  Optional supplementation of non-essential amino acids to the formulations that incorporate either Hanks' or Eagles' salts has broadened the usefulness of this medium.


Directions per Liter Dissolve 15.3 grams per liter of distilled/deionized (DDI) water, heating with stirring until completely solubilized, adjusting pH as necessary. If required, add sodium bicarbonate. Dispense into appropriate containers, loosen caps and autoclave for 15-30 minutes at 121ºC (15psi).

Storage Store at 0-5ºC (store unopened media at 2-30°C). Opened bottles should be capped tightly and kept in a dark, low humidity environment.


Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

1. Eagle, H. et al., J. Biol. Chem. 214:845-847 (1956)
2. Eagle, H. Tissue Culture Association Manual 3:517-520 (1976)
3. Eagle, H. Science 130:432-437 (1959).
4. Eagle, H. Science 122:501 (1955)
5. Stanners, C.P., Eliceiri, G.L. and Green, H. Nature New Biology 230:52-54 (1971)
6. Stanners, C.P. and Goldberg, V. J., J. Gen. Virol. 29:81-296. (1975)