Technical Data
Media
MEM Eagle w/ Hanks’, w/o L-Glutamine, Non-essential Amino Acids (Powder)
M3864-05
Cell Culture Grade
Storage RT    Shipping RT
Components shown as mg/liter
Inorganic Salts:
Calcium Chloride•2H2O185.50
Potassium Chloride400.00
Potassium Phosphate Mono60.00
Magnesium Sulfate, anhy 97.70
Sodium Chloride8000.00
Sodium Phosphate Dibasic47.50
Amino Acids:
L-Arginine126.40
L-Cystine, anhy24.02
L-Histidine•HCl41.90
L-Isoleucine52.50
L-Leucine52.50
L-Lysine•HCI73.06
L-Methionine14.90
L-Phenylalanine33.02
L-Threonine47.64
L-Tryptophan10.20
L-Tyrosine, anhy 36.22
L-Valine46.90
Vitamins:
D-Pantothenic Acid•Ca1.00
Choline Chloride1.00
Folic Acid1.00
myoi-Inositol2.00
Nicotinamide1.00
Pyridoxal•HCI1.00
Riboflavin0.10
Thiamine•HCI1.00
Other:
D-Glucose1000.00
Phenol Red, Sodium17.00
Total:10.375g/liter
Minimum Essential Medium (MEM), developed by Harry Eagle, is one of the most widely used of all synthetic cell culture media.  Early attempts to cultivate normal mammalian fibroblasts and certain subtypes of HeLa cells revealed that they had specific nutritional requirements that could not be met by Eagle's Basal Medium (BME). Subsequent studies using these and other cells in culture indicated that additions to BME could be made to aid growth of a wider variety of fastidious cells. MEM, which incorporates these modifications, includes higher concentrations of amino acids so that the medium more closely approximates the protein composition of mammalian cells. MEM has been used for cultivation of a wide variety of cells grown in monolayers.  Optional supplementation of non-essential amino acids to the formulations that incorporate either Hanks' or Eagles' salts has broadened the usefulness of this medium.


Directions per Liter Dissolve 10.4 grams per liter of distilled/deionized (DDI) water, heating with stirring until completely solubilized, adjusting pH as necessary. If required, add sodium bicarbonate. Dispense into appropriate containers, loosen caps and autoclave for 15 minutes at 121ºC (15psi).

Storage Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept refrigerated and used within a short period of time.


Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

1. Eagle, H. et al., J. Biol. Chem. 214:845-847 (1956)
2. Eagle, H. Tissue Culture Association Manual 3:517-520 (1976)
3. Eagle, H. Science 130:432-437 (1959).
4. Eagle, H. Science 122:501 (1955)
5. Stanners, C.P., Eliceiri, G.L. and Green, H. Nature New Biology 230:52-54 (1971)
6. Stanners, C.P. and Goldberg, V. J., J. Gen. Virol. 29:81-296. (1975)