Technical Data
Media
MEM Eagle Joklik Suspension Modification w/L-Glutamine w/o Calcium Chloride (Powder)
M3867
Cell Culture Grade
Storage RT/4°C    Shipping RT
Components shown as g/liter
Inorganic Salts:
Magnesium Chloride, anhy0.094
Potassium Chloride0.400
Sodium Chloride6.500
Sodium Phosphate Monobasic 1.154
Amino Acids:
L-Arginine•HCl0.126
L-Cystine•2HCl0.0324
L-Glutamine0.292
L-Histidine•HCl•H2O0.042
L-Isoleucine0.052
L-Leucine0.052
L-Lysine•HCl0.0725
L-Methionine0.015
L-Phenylalanine0.032
L-Threonine0.048
L-Tryptophan0.010
L-Tyrosine•2Na•2H2O0.05452
L-Valine
Vitamins:
Choline Chloride0.001
Folic Acid0.001
myo-Inositol0.002
Niacinamide0.001
D-Pantothenic Acid•Ca0.001
Pyridoxal•HCl0.001
Riboflavin0.0001
Thiamie•HCl0.001
Other:
D-Glucose2.000
Phenol Red, Sodium0.011
Total:11.042g/L
Minimum Essential Medium (MEM) with Earle’s Balanced Salts was developed by Harry Eagle as a result of his studies to determine the essential nutritional requirements of mouse L cells and HeLa cells in culture. He demonstrated that these cells require 13 amino acids and 7 vitamins to grow and reproduce in vitro. MEM is a modification of Eagle’s earlier medium Basal Medium Eagle (BME), containing higher concentrations of the essential nutrients. This medium has demonstrated the ability to support a variety of normal and transformed cells in culture and contains Earle’s Balanced Salts, which makes it suitable for use in atmospheres charged with CO2 gas. Minimum Essential Medium Joklik’s Modification (JMEM) was designed for suspension culture systems. Calcium chloride has been omitted to reduce cellular attachment.


Directions per Liter: Dissolve 11.042g in 800-900ml of ddH2O, stirring gently until completely solubilized. If required, add 2.2g sodium bicarbonate with stirring. Adjust pH of the medium to 0.1-0.3 pH unit below the desired level. Add additional water to bring the solution to 1L. Filter-sterilize using a 0.22 micron membrane filter. Aliquot into sterile containers. Do not autoclave. Contains heat-labile compounds that can be damaged with autoclaving.

Storage and Stability: Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.

Appearance: Yellow to orange, homogenous, free flowing powder.

Solubility: Deep yellow, clear, complete

pH: As reported

Endotoxin: 1EU/ml


Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

1. Eagle, H., et al., J. Biol. Chem. 214: 845-847 (1956). 2. Eagle, H., Tissue Culture Association Manual 3: 517-520 (1976). 3. Eagle, H., Science 130: 432-437 (1959). 4. Eagle, H., Science 122: 501 (1955).