Technical Data
Media
MEM Eagle Suspension Modification w/L-Glutamine (Powder)
M3869
Cell Culture Grade
Storage RT    Shipping RT
Components shown as g/liter
Inorganic Salts:
MagnesiumSulfate0.098
Potassium Chloride0.400
Potassium Phosphate Monob0.060
Sodium Chloride6.800
Sodium Phosphate Dibasic0.048
Amino Acids:
L-Alanine0.009
L-Arginine0.127
L-Cystine•2HCI0.031
L-Glutamine0.292
L-Histidine•HCl0.042
L-Isoleucine0.052
L-Leucine0.052
L-Lysine•HCI0.073
L-Methionine0.015
L-Phenylalanine0.033
L-Threonine0.048
L-Tryptophan0.008
L-Tyrosine•2Na0.052
L-Valine0.046
Vitamins:
Choline Chloride0.001
Folic Acid0.001
i-Inositol0.002
Nicotinamide0.001
D-Pantothenic Acid•Ca0.001
Pyridoxal•HCI0.001
Riboflavin0.0002
Thiamine•HCI0.001
Other:
D-Glucose1.000
Phenol Red, Sodium0.015
TOTAL9.309
Minimum Essential Medium (MEM), developed by Harry Eagle, is one of the most widely used of all synthetic cell culture media.  Early attempts to cultivate normal mammalian fibroblasts and certain subtypes of HeLa cells revealed that they had specific nutritional requirements that could not be met by Eagle's Basal Medium (BME). Subsequent studies using these and other cells in culture indicated that additions to BME could be made to aid growth of a wider variety of fastidious cells. MEM, which incorporates these modifications, includes higher concentrations of amino acids so that the medium more closely approximates the protein composition of mammalian cells. MEM has been used for cultivation of a wide variety of cells grown in monolayers.  Optional supplementation of non-essential amino acids to the formulations that incorporate either Hanks' or Eagles' salts has broadened the usefulness of this medium.

Appearance:
Light pink, homogenous, free flowing

Solubility:
Magenta, clear, complete

pH:
As reported

Endotoxin:
10Eu/ml


Directions per Liter: Dissolve 9.31grams in 900ml of ddH2O, stirring gently until completely solubilized. Do NOT heat. If required, add 2grams sodium bicarbonate with stirring. Adjust pH of the medium to 0.1-0.3 pH units below the desired pH. Add additional water to bring the solution to final volume. Filter-sterilize using a 0.22 micron filter. Aliquot into sterile containers. Do not autoclave. Contains heat-labile compounds that can be damaged with autoclaving.

Storage and Stability: Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.


Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

1. Eagle, H. et al., J. Biol. Chem. 214:845-847 (1956) 2. Eagle, H. Tissue Culture Association Manual 3:517-520 (1976) 3. Eagle, H. Science 130:432-437 (1959). 4. Eagle, H. Science 122:501 (1955) 5. Stanners, C.P., Eliceiri, G.L. and Green, H. Nature New Biology 230:52-54 (1971) 6. Stanners, C.P. and Goldberg, V. J., J. Gen. Virol. 29:81-296. (1975)