Technical Data
Media
MEM Eagle Suspension Modification w/L-Glutamine, HEPES (Powder)
M3870
Cell Culture Grade
Storage RT/4°C    Shipping RT
Components shown as mg/liter
Inorganic Salts:
Magnesium Sulfate97.67
Potassium Chloride400
Potassium Phosphate Mono60
Sodium Chloride8000
Sodium Phosphate Dibasic47.69
Amino Acids:
L-Alanine8.9
L-Arginine126.98
L-Cystine•2HCI31.29
L-Glutamine292
L-Histidine•HCl42
L-Isoleucine52
L-Leucine52
L-Lysine•HCI73.1
L-Methionine15
L-Phenylalanine33
L-Threonine48
L-Tryptophan8
L-Tyrosine•2Na•2H2051.9
L-Valine46
Vitamins:
Choline Chloride1
Folic Acid1
i-Inositol2
Nicotinamide1
D-Pantothenic Acid•Ca1
Pyridoxal•HCI1
Riboflavin0.2
Thiamine•HCI1
Other:
D-Glucose1000
HEPES Free Acid5958
Phenol Red, Sodium15.3
Total:16.47g/liter
Minimum Essential Medium (MEM), developed by Harry Eagle, is one of the most widely used of all synthetic cell culture media.  Early attempts to cultivate normal mammalian fibroblasts and certain subtypes of HeLa cells revealed that they had specific nutritional requirements that could not be met by Eagle's Basal Medium (BME). Subsequent studies using these and other cells in culture indicated that additions to BME could be made to aid growth of a wider variety of fastidious cells. MEM, which incorporates these modifications, includes higher concentrations of amino acids so that the medium more closely approximates the protein composition of mammalian cells. MEM has been used for cultivation of a wide variety of cells grown in monolayers.  Optional supplementation of non-essential amino acids to the formulations that incorporate either Hanks' or Eagles' salts has broadened the usefulness of this medium.


Directions per Liter: Dissolve 16.47g in 800-900ml of ddH2O stirring gently until completely solubilized. Add 2.0g/L sodium bicarbonate. Adjust pH of the medium to the desired level. Add additional water to bring the solution to1L Filter-sterilize using a 0.22 micron membrane filter. Aliquot into sterile containers. Do not autoclave. Contains heat-labile compounds that can be damaged with autoclaving.

Storage and Stability: Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.

Appearance: Red to orange, homogenous, free flowing powder

Solubility: Pink to light orange, clear, complete

pH: As reported

Endotoxin: 10Eu/mg


Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

1. Eagle, H. et al., J. Biol. Chem. 214:845-847 (1956) 2. Eagle, H. Tissue Culture Association Manual 3:517-520 (1976) 3. Eagle, H. Science 130:432-437 (1959). 4. Eagle, H. Science 122:501 (1955) 5. Stanners, C.P., Eliceiri, G.L. and Green, H. Nature New Biology 230:52-54 (1971) 6. Stanners, C.P. and Goldberg, V. J., J. Gen. Virol. 29:81-296. (1975)