Technical Data
Media
MEM w/Earle’s Temin’s Modification w/L-Glutamine, NaHCO3, antibiotics (Powder)
M3873
Cell Culture Grade
Storage RT    Shipping RT
Components shown as mg/liter
Inorganic Salts:
Sodium Chloride6080
Potassium Chloride400
Magnesium Sulfate100
Sodium Phosphate Monobasic121.4
FerricNitrate0.1
Calcium Chloride199.59
Amino Acids:
L-Arginine•HCl253.96
L-Cystine•2Na51.01
L-Glutamine584
Glycine30
L-Histidine•HCl83.76
L-Isoleucine105
L-Leucine105
L-Lysine•HCI145
L-Methionine30
L-Phenylalanine64
L-Serine42
L-Threonine94
L-Tryptophan20
L-Tyrosine•2Na103.79
L-Valine96
Vitamins:
D-Pantothenic Acid•Ca2
Choline Chloride2
FolicAcid2
i-Inositol4
Nicotinamide2
PyridoxalHCI2
Riboflavin0.2
Thiamine•HCl2
Antibiotics:
Streptomycin Sulfate (~750mcg/mg)104.2
Penicillin G•Na (~1600u/mg)60.1
Other:
D-Glucose4500
Phenol Red, Sodium10
SodiumBicarbonate4200
Total17.4g/liter



Directions per Liter: 1. Add 900ml distilled/deionized water (25ºC) to a 1 liter container.2. Weigh 17.6g into container, stirring gently until completely solubilized. Heat gently with stirring.

3. Add 2.0g/Liter NaHCO3 with stirring. 4. Adjust pH to ± 0.1-0.3 pH units with 1N HCl or 1N NaOH.5. q.s. to final 1 Liter volume.6. Sterilize with 0.2µ membrane filter. Do not autoclave since heat-labile vitamins will lose their activity.7. Aliquot into sterile containers, store at 0-5ºC. Keep away from light.


Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

1. Eagle, H. et al., J. Biol. Chem. 214:845-847 (1956)
2. Eagle, H. Tissue Culture Association Manual 3:517-520 (1976)
3. Eagle, H. Science 130:432-437 (1959).
4. Eagle, H. Science 122:501 (1955)
5. Stanners, C.P., Eliceiri, G.L. and Green, H. Nature New Biology 230:52-54 (1971)
6. Stanners, C.P. and Goldberg, V. J., J. Gen. Virol. 29:81-296. (1975)