Technical Data
Media
MEM w/Earle’s Temin’s Modification w/L-Glutamine, NaHCO3, antibiotics (Powder)
M3873
Cell Culture Grade
Storage 4°C    Shipping RT
Components shown as mg/liter
Inorganic Salts:
Calcium Chloride199.59
Ferric Nitrate0.1
Magnesium Sulfate100
Potassium Chloride400
Sodium Chloride6080
Sodium Phosphate Monobasic121.4
Amino Acids:
L-Arginine•HCl253.96
L-Cystine•2Na51.01
L-Glutamine584
Glycine30
L-Histidine•HCl83.76
L-Isoleucine105
L-Leucine105
L-Lysine•HCI145
L-Methionine30
L-Phenylalanine64
L-Serine42
L-Threonine94
L-Tryptophan20
L-Tyrosine•2Na103.79
L-Valine96
Vitamins:
Choline Chloride2
Folic Acid2
i-Inositol4
Nicotinamide2
D-Pantothenic Acid•Ca2
Pyridoxal•HCI2
Riboflavin0.2
Thiamine•HCl2
Antibiotics:
Penicillin G•Na (~1600u/mg)60.1
Streptomycin Sulfate (~750mcg/mg)104.2
Other:
D-Glucose4500
Phenol Red, Sodium10
Sodium Bicarbonate4200
Total17.6g/liter
Appearance: Light pink to light orange, clear, complete

Solubility: Red, clear, complete

pH: As Reported


Directions per Liter: Dissolve 17.6g in 800-900ml of ddH2O stirring gently until completely solubilized. Add additional water to bring the solution to1L Filter-sterilize using a 0.22 micron membrane filter. Aliquot into sterile containers. Do not autoclave. Contains heat-labile compounds that can be damaged with autoclaving.

Storage and Stability: Store powdered media at 4ºC. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.


Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

1. Eagle, H. et al., J. Biol. Chem. 214:845-847 (1956). 2. Eagle, H. Tissue Culture Association Manual 3:517-520 (1976). 3. Eagle, H. Science 130:432-437 (1959). 4. Eagle, H. Science 122:501 (1955)
5. Stanners, C.P., Eliceiri, G.L. and Green, H. Nature New Biology 230:52-54 (1971). 6. Stanners, C.P. and Goldberg, V. J., J. Gen. Virol. 29:81-296. (1975)