Technical Data

Media

MEM w/Earle’s Temin’s Modificationw/L-Glutamine, NaHCO3, antibiotics (Powder)

M3873

Cell Culture Grade

Storage RT Shipping RT

Components shown as mg/liter


Inorganic Salts:
Sodium Chloride	6080
Potassium Chloride	400
Magnesium Sulfate	100
Sodium Phosphate Monobasic	121.4
FerricNitrate	0.1
Calcium Chloride	199.59

Amino Acids:
L-Arginine•HCl	253.96
L-Cystine•2Na	51.01
L-Glutamine	584
Glycine	30
L-Histidine•HCl	83.76
L-Isoleucine	105
L-Leucine	105
L-Lysine•HCI	145
L-Methionine	30
L-Phenylalanine	64
L-Serine	42
L-Threonine	94
L-Tryptophan	20
L-Tyrosine•2Na	103.79
L-Valine	96

Vitamins:
D-Pantothenic Acid•Ca	2
Choline Chloride	2
FolicAcid	2
i-Inositol	4
Nicotinamide	2
PyridoxalHCI	2
Riboflavin	0.2
Thiamine•HCl	2

Antibiotics:
Streptomycin Sulfate (~750mcg/mg)	104.2
Penicillin G•Na (~1600u/mg)	60.1

Other:
D-Glucose	4500
Phenol Red, Sodium	10
SodiumBicarbonate	4200

Total	17.4g/liter

Directions per Liter: 1. Add 900ml distilled/deionized water (25ºC) to a 1 liter container.2. Weigh 17.6g into container, stirring gently until completely solubilized. Heat gently with stirring.

3. Add 2.0g/Liter NaHCO3 with stirring. 4. Adjust pH to ± 0.1-0.3 pH units with 1N HCl or 1N NaOH.5. q.s. to final 1 Liter volume.6. Sterilize with 0.2µ membrane filter. Do not autoclave since heat-labile vitamins will lose their activity.7. Aliquot into sterile containers, store at 0-5ºC. Keep away from light.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

1. Eagle, H. et al., J. Biol. Chem. 214:845-847 (1956)
2. Eagle, H. Tissue Culture Association Manual 3:517-520 (1976)
3. Eagle, H. Science 130:432-437 (1959).
4. Eagle, H. Science 122:501 (1955)
5. Stanners, C.P., Eliceiri, G.L. and Green, H. Nature New Biology 230:52-54 (1971)
6. Stanners, C.P. and Goldberg, V. J., J. Gen. Virol. 29:81-296. (1975)